Surface Enhanced Raman Scattering Probes Based onTargeted Gold Nanorods for Detecting Hela Cells

FENG Yu-qing; LI Xi

doi:10.3788/fgxb20194010.1215

您当前的位置：

首页 >

文章列表页 >

Surface Enhanced Raman Scattering Probes Based onTargeted Gold Nanorods for Detecting Hela Cells

Synthesis and Properties of Materials | 更新时间：2020-08-12

- Surface Enhanced Raman Scattering Probes Based onTargeted Gold Nanorods for Detecting Hela Cells
- Chinese Journal of Luminescence Vol. 40, Issue 10, Pages: 1215-1219(2019)
- 作者机构：
  
  长春工业大学化学与生命科学学院,吉林长春,130012
- 作者简介：
- 基金信息：
  
  T Development Plan Project of China (20160414043GH);13th Five-Year"
- DOI：10.3788/fgxb20194010.1215
  CLC： Q599
- Received：27 May 2019，
  
  Revised：30 June 2019，
  
  Published：05 October 2019
- 稿件说明：
移动端阅览
冯雨晴, 李奚,. 基于SERS技术的靶向修饰金纳米探针用于Hela细胞的检测[J]. 发光学报, 2019,40(10): 1215-1219

FENG Yu-qing, LI Xi,. Surface Enhanced Raman Scattering Probes Based onTargeted Gold Nanorods for Detecting Hela Cells[J]. Chinese Journal of Luminescence, 2019,40(10): 1215-1219
冯雨晴, 李奚,. 基于SERS技术的靶向修饰金纳米探针用于Hela细胞的检测[J]. 发光学报, 2019,40(10): 1215-1219 DOI： 10.3788/fgxb20194010.1215.

FENG Yu-qing, LI Xi,. Surface Enhanced Raman Scattering Probes Based onTargeted Gold Nanorods for Detecting Hela Cells[J]. Chinese Journal of Luminescence, 2019,40(10): 1215-1219 DOI： 10.3788/fgxb20194010.1215.

摘要

表皮生长因子受体（EGFR）是一种肿瘤表面标记性蛋白。本文报道了基于anti-EGFR功能化金纳米棒探针AuNRs probes的表面增强拉曼散射（SERS），用于EGFR阳性肿瘤细胞的检测。通过AuNRs probes上anti-EGFR特异性结合到EGFR阳性癌细胞上，可使修饰于金纳米棒表面的拉曼活性染料4-巯基苯甲酸（4-MBA）位于1 100 cm

-1

和1 600 cm

-1

的特征峰强度得到信号增强。该SERS探针由于具有生物兼容性好、细胞拉曼信号稳定、特异性高等优点而具有巨大的临床应用前景。

Abstract

Epidermal growth factor receptor(EGFR)is overexpressed on tumor surface. A surface enhanced Raman scattering(SERS) probe based on epidermal growth factor receptor antibody(anti-EGFR) conjugated Au nanorods(AuNRs probes) was used for EGFR positive cancer cell detection. When AuNRs probes were specific bound to EGFR positive cell

SERS signals from Raman active molecule 4-mercaptobenzoic acid(4-MBA) modified on AuNRs were two to three times more than those cells incubated with 4-MBA labeled AuNRs(AuNRs-MBA). This kind of SERS probe has great potential in the diagnosis of EGFR positive tumor due to its specific targeting

high SERS sensitivity and biocompatibility.

关键词

Keywords

references

HALDAVNEKAR R,VENKATAKRISHNAN K,TAN B. Non plasmonic semiconductor quantum SERS probe as a pathway for in vitro cancer detection[J]. Nat. Commun., 2018,9(1):3065-1-18.

HUANG Z W,MCWILLIAMS A,LUI H,et al.. Near-infrared Raman spectroscopy for optical diagnosis of lung cancer[J]. Int. J. Cancer, 2003,107(6):1047-1052.

LI J B,CHENG W N,WANG X L,et al.. Electron transfer of cytochrome c on surface-enhanced Raman scattering-active substrates:material dependence and biocompatibility[J]. Chemistry, 2017,23(38):9034-9038.

ZOU S,WEAVER M J. Surface-enhanced Raman scattering on uniform transition-metal films:toward a versatile adsorbate vibrational strategy for solid-nonvacuum interfaces[J]. Anal. Chem., 1998,70(11):2387-2395.

QIAO X Z,SU B S,LIU C,et al.. Selective surface enhanced raman scattering for quantitative detection of lung cancer biomarkers in superparticle@MOF structure[J]. Adv. Mater., 2018,30(5):1702275.

ANDREOU C,KISHORE S A,KIRCHER M F. Surface-enhanced raman spectroscopy:a new modality for cancer imaging[J]. J. Nucl. Med., 2015,56(9):1295-1299.

BUTLER H J,FOGARTY S W,KERNS J G,et al.. Gold nanoparticles as a substrate in bio-analytical near-infrared surface-enhanced Raman spectroscopy[J]. Analyst, 2015,140(9):3090-3097.

ALTUNBEK M,KUKU G,CULHA M. Gold nanoparticles in single-cell analysis for surface enhanced raman scattering[J]. Molecules, 2016,21(12):1617-1-18.

DOKALA A,THAKUR S S. Extracellular region of epidermal growth factor receptor:a potential target for anti-EGFR drug discovery[J]. Oncogene, 2017,36(17):2337-2344.

COSTA R,SHAH A N,SANTA-MARIA C A,et al.. Targeting epidermal growth factor receptor in triple negative breast cancer:new discoveries and practical insights for drug development[J]. Cancer Treat. Rev., 2017,53:111-119.

YEWALE C,BARADIA D,VHORA I,et al.. Epidermal growth factor receptor targeting in cancer:a review of trends and strategies[J]. Biomaterials, 2013,34(34):8690-8707.

KEUL H A,MLLER M,BOCKSTALLER M R. Structural evolution of gold nanorods during controlled secondary growth[J]. Langmuir, 2007,23(20):10307-10315.

BRIOUDE A,JIANG X C,PILENI M P. Optical properties of gold nanorods:DDA simulations supported by experiments[J]. J. Phys. Chem. B, 2005,109(27):13138-13142.

WILLITSFORD A H,CHADWICK C T,KURTZ S,et al.. Resonance-enhanced raman scattering of ring-involved vibrational modes in the 1B2u absorption band of benzene,including the kekule vibrational modes 9 and 10[J]. J. Phys. Chem. A, 2016,120(4):503-506.

Views

218

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Synthesization and Toxicity of Low-molecular-weight PEI/Gold Nanords Nanosystem

Passively Q-switched Er-doped Fiber Lasers by Using Gold Nanorods as Saturable Absorbers

Related Author

WANG Qian

LIU Li-wei

ZHU Ling-xi

ZOU Peng

HU Si-yi

BU Cheng-fei

XU Yang

KANG Zhe

Related Institution

International Joint Research Center for Nanophotonics and Biophotonics, School of Science, Changchun University of Science and Technology

集成光电子学国家重点联合实验室吉林大学实验区吉林大学电子科学与工程学院

AI问答

Address：No.3888 Dong Nanhu Road, Changchun, Jilin, China Postal code：130033
Tel：0431-86176862 Email：fgxbt@126.com
Technical support is provided by Beijing Founder electronics co., LTD 吉ICP备11002662号-17 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰