ZHOU Jin, ZHANG Mei-ling, ZHANG Li etc. Studies of Solid Phase Immunoassay Based on Near Infrared Upconversion Nanoprobe[J]. Chinese Journal of Luminescence, 2018,39(8): 1059-1065
ZHOU Jin, ZHANG Mei-ling, ZHANG Li etc. Studies of Solid Phase Immunoassay Based on Near Infrared Upconversion Nanoprobe[J]. Chinese Journal of Luminescence, 2018,39(8): 1059-1065 DOI: 10.3788/fgxb20183908.1059.
Studies of Solid Phase Immunoassay Based on Near Infrared Upconversion Nanoprobe
nanoparticles were synthesized through solvent thermal method in chloride. PAA-based ligand replace strategies were carried out to introduce free carboxylic acid groups which allowed further conjugation with NH
2
-containing human IgG. The silicon wafer was modified with rabbit anti-goat IgG by using protein G as "bridge"
which contributed to eliminate structural or conformational changes of IgG. Owing to the specific antibody recognition to the antigenic
a simple
rapid
efficient and sensitive immunoassay method was developed. The experimental results demonstrate that there is a very good linear correlation between the upconversion luminescence intensity and the amount of goat anti-human IgG in the range of 5~400 nmol/L. The detection limit is as low as 1.82 nmol/L. Our developed detection system shows excellent detection specificity and sensitivity
which provides a new detection method and platform with wide linear range and high sensitivity for diagnosis of major clinical diseases in the future.
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references
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