ZENG Yun-long, CHENG Yuan-xin, YI Shou-jun etc. A Novel Aptasensor Assay for Aflatoxin B1 in Traditional Chinese Medicine[J]. Chinese Journal of Luminescence, 2018,39(4): 589-594
ZENG Yun-long, CHENG Yuan-xin, YI Shou-jun etc. A Novel Aptasensor Assay for Aflatoxin B1 in Traditional Chinese Medicine[J]. Chinese Journal of Luminescence, 2018,39(4): 589-594 DOI: 10.3788/fgxb20183904.0589.
A Novel Aptasensor Assay for Aflatoxin B1 in Traditional Chinese Medicine
The nanocomposites of Au nanoparticles/aptamers/quantum dots were constructed by self-assembly. Fluorescence of CdTe quantum dots can be quenched by Au nanoparticles in the nanocomposites. The fluorescence of the system can recover when aflatoxin B1 (AFB1) is added to the assay solution
specific interactions between AFB1 and the aptamer cause release of the quantum dots. A linear fluorescence signal response to AFB1 concentration is obtained over a wide AFB1 concentration range of 0.005-2.00 ng/mL with a detection limit of 1.2 pg/mL. The proposed method has been employed in the determination of trace AFB1 in forsythia suspense
hawthorn and radix liquiritiae successfully.
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