SHEN Bing-jun, LIU Zhan-wei, LIU Rong-juan etc. Study on Mechanism of Interactions Between DNA and Corilagin by Spectroscopy[J]. Chinese Journal of Luminescence, 2017,38(12): 1681-1687
SHEN Bing-jun, LIU Zhan-wei, LIU Rong-juan etc. Study on Mechanism of Interactions Between DNA and Corilagin by Spectroscopy[J]. Chinese Journal of Luminescence, 2017,38(12): 1681-1687 DOI: 10.3788/fgxb20173812.1681.
Study on Mechanism of Interactions Between DNA and Corilagin by Spectroscopy
using ethidium bromide (EB) as the fluorescent probe
the interaction mechanism between coriolis (Cor) and calf thymus DNA was studied by UV-Vis absorption spectroscopy
resonance scattering spectroscopy combined with salt effect and DNA melting point. Cor can quench the fluorescence of EB-DNA system through a static process. The subtractive effect of the characteristic absorption peak occurrs after Cor interacts with DNA. The interaction leads to the enhancement of Cor resonance at 481 nm and a new resonance scattering peak at 330 nm. The salt effect has a little influence on the interaction between Cor and DNA. The interaction leads to the increase of the
T
m
of DNA by 5.5℃. It is deduced that the main way of the interaction between Cor and DNA is intercalation
and a supramolecular system is formed between them. The binding constant (
K
A
) between Cor and DNA is 5.8210
3
L/mol (298 K) and 2.4710
4
L/mol (310 K). The interaction between Cor and DNA is entropy-driven spontaneous
endothermic. Hydrophobic forces are the main non-covalent mode of action.
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references
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