InP@ZnS QDs/Dured Fluorescent Nanoprobe for The Detection of DNA

HU Xian-yun; MENG Tie-hong; ZHANG Ru-guo; JIANG Jia-zhi; HUANG Xing-hong

doi:10.3788/fgxb20173803.0288

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InP@ZnS QDs/Dured Fluorescent Nanoprobe for The Detection of DNA

更新时间：2020-08-12

- InP@ZnS QDs/Dured Fluorescent Nanoprobe for The Detection of DNA
- Chinese Journal of Luminescence Vol. 38, Issue 3, Pages: 288-295(2017)
- 作者机构：
  
  1. 东南大学生物科学与医学工程学院生物电子学国家重点实验室, 江苏南京 210096
  2. 黔南民族医学高等专科学校, 贵州都匀 558000
- 作者简介：
- 基金信息：
  
  Supported by National Natural Science Foundation of China(21545006);Natural Scienc
- DOI：10.3788/fgxb20173803.0288
  CLC： O482.31
- Received：19 September 2016，
  
  Revised：15 October 2016，
  
  Published：05 March 2017
- 稿件说明：
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胡先运, 孟铁宏, 张汝国等. 基于InP@ZnS QDs/Dured纳米荧光探针的DNA检测[J]. 发光学报, 2017,38(3): 288-295

HU Xian-yun, MENG Tie-hong, ZHANG Ru-guo etc. InP@ZnS QDs/Dured Fluorescent Nanoprobe for The Detection of DNA[J]. Chinese Journal of Luminescence, 2017,38(3): 288-295
胡先运, 孟铁宏, 张汝国等. 基于InP@ZnS QDs/Dured纳米荧光探针的DNA检测[J]. 发光学报, 2017,38(3): 288-295 DOI： 10.3788/fgxb20173803.0288.

HU Xian-yun, MENG Tie-hong, ZHANG Ru-guo etc. InP@ZnS QDs/Dured Fluorescent Nanoprobe for The Detection of DNA[J]. Chinese Journal of Luminescence, 2017,38(3): 288-295 DOI： 10.3788/fgxb20173803.0288.

摘要

利用巯基丙酸包覆的InP@ZnS量子点（QDs）与Dured构建了一种检测DNA的荧光探针。在该探针中，以环境友好型带负电的InP@ZnS量子点为荧光团，与带正电的Dured通过静电结合，构建了InP@ZnS QDs/Dured纳米荧光探针。通过荧光共振能量转移（FRET）机理，量子点荧光被猝灭；当DNA存在时，Dured与DNA的特异性结合使Dured从InP@ZnS QDs表面脱附，FRET过程被打断，InP@ZnS QDs荧光恢复，以荧光关-开方式检测DNA。该探针检测DNA的线性范围为2.0~275.0 ngL

-1

，检测限为1.0 ngL

-1

，并可用于模拟生物生理条件下的DNA检测。

Abstract

The fluorescent nanoprobe for the detection of DNA was established by utilizing mercaptopropionic acid coated InP@ZnS QDs and Dured. In this nanoprobe. The InP@ZnS QDs/Dured fluorescent nanoprobes were structured by electrostatic interactions between environment friendly

negatively charged InP@ZnS quantum dots and positively charged Dured

and then the fluorescence of InP@ZnS QDs/Dured was quenched through fluorescence resonance energy transfer (FRET). At presence of DNA

DNA and Dured were specific binding

which enable Dured removing from the surface of InP@ZnS QDs and achieving fluorescence recovery of InP@ZnS QDs. Thus

the detection of DNA was realized. The linear range of the InP@ZnS QDs/Dured fluorescent nanoprobes for DNA detection and the detection limit were 2.0-275.0 ngL

-1

and 1.0 ngL

-1

respectively. The InP@ZnS QDs/Dured fluorescent nanoprobes can be also used in rapid detection of DNA under simulated physiological conditions.

关键词

Keywords

references

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