Detection of Ascorbic Acid by Fluorescence Probe Based on Graphene Quantum Dots

ZHAO Li-min; CHEN Li-ni; ZHAO Shu-lin

doi:10.3788/fgxb20173801.0124

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Detection of Ascorbic Acid by Fluorescence Probe Based on Graphene Quantum Dots

更新时间：2020-08-12

- Detection of Ascorbic Acid by Fluorescence Probe Based on Graphene Quantum Dots
- Chinese Journal of Luminescence Vol. 38, Issue 1, Pages: 124-131(2017)
- 作者机构：
  
  1. 安阳市质量技术监督检验测试中心,河南安阳,455000
  2. 广西师范大学化学与药学学院教育部药用资源化学与药物分子工程重点实验室,广西桂林,541004
- 作者简介：
- 基金信息：
  
  Supported by Natural Science Foundation of China(21305020,21175030);Natural Scienc
- DOI：10.3788/fgxb20173801.0124
  CLC： O482.31
- Received：23 June 2016，
  
  Revised：23 September 2016，
  
  Published：05 January 2017
- 稿件说明：
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赵丽敏, 陈丽妮, 赵书林. 基于石墨烯量子点的荧光探针应用于抗坏血酸检测的研究[J]. 发光学报, 2017,38(1): 124-131

ZHAO Li-min, CHEN Li-ni, ZHAO Shu-lin. Detection of Ascorbic Acid by Fluorescence Probe Based on Graphene Quantum Dots[J]. Chinese Journal of Luminescence, 2017,38(1): 124-131
赵丽敏, 陈丽妮, 赵书林. 基于石墨烯量子点的荧光探针应用于抗坏血酸检测的研究[J]. 发光学报, 2017,38(1): 124-131 DOI： 10.3788/fgxb20173801.0124.

ZHAO Li-min, CHEN Li-ni, ZHAO Shu-lin. Detection of Ascorbic Acid by Fluorescence Probe Based on Graphene Quantum Dots[J]. Chinese Journal of Luminescence, 2017,38(1): 124-131 DOI： 10.3788/fgxb20173801.0124.

摘要

基于石墨烯量子点（GQDs）的荧光性能建立了一种非标记荧光方法，用于灵敏和选择性测定抗坏血酸（AA）。GQDs溶液在紫外光激发下发出很强的蓝色荧光，当向溶液中加入AA后，GQDs溶液的荧光被猝灭。猝灭机理可能为在弱酸性介质中，AA与GQDs发生氧化还原反应，AA转移电子给GQDs。荧光猝灭强度与AA浓度在5.010

-6

~7.510

-5

mol/L范围内呈良好的线性关系，检出限低至1.010

-6

mol/L。该体系成本低、操作简单，并且在多种可能干扰的物质存在下对AA表现出很高的选择性。本方法应用于生物样品中AA的检测，回收率在95.2%~115.3%之间。

Abstract

A label-free fluorescence method based on the fluorescence property of graphene quantum dots (GQDs) was developed for sensitive and selective detection of ascorbic acid (AA). The initial strong blue fluorescence of the GQDs in aqueous solution was effectively quenched upon addition of AA. The quenching mechanism may involve transfer of electrons from AA to GQDs

via

the redox reaction of AA and GQDs in weak acid solution. The quenching efficiency was linearly proportional to the concentration of AA within the range of 5.010

-6

-7.510

-5

mol/L with a low detection limit down to 1.010

-6

mol/L. The proposed sensing system is simple and low-cost with facile experimental operations

and has a high selectivity for AA over a number of possible interfering species. Additionally

this method was successfully applied to the determination of AA in biological samples with satisfactory recoveries (95.2%-115.3%).

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references

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