Fluorescent Aptamer Biosensor for The Detection of Dopamine with Graphene Oxide

JIANG Li-ying; ZHOU Peng-lei; XIAO Xiao-nan; ZHANG Pei; LIU Shuai; YAN Yan-xia; CHEN Qing-

doi:10.3788/fgxb20163707.0881

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Fluorescent Aptamer Biosensor for The Detection of Dopamine with Graphene Oxide

更新时间：2020-08-12

- Fluorescent Aptamer Biosensor for The Detection of Dopamine with Graphene Oxide
- Chinese Journal of Luminescence Vol. 37, Issue 7, Pages: 881-886(2016)
- 作者机构：
  
  郑州轻工业学院电气信息工程学院, 河南郑州 450002
- 作者简介：
- 基金信息：
- DOI：10.3788/fgxb20163707.0881
  CLC： TP212
- Received：29 February 2016，
  
  Revised：24 March 2016，
  
  Published：05 July 2016
- 稿件说明：
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姜利英, 周鹏磊, 肖小楠等. 基于氧化石墨烯荧光适体传感器的多巴胺检测[J]. 发光学报, 2016,37(7): 881-886

JIANG Li-ying, ZHOU Peng-lei, XIAO Xiao-nan etc. Fluorescent Aptamer Biosensor for The Detection of Dopamine with Graphene Oxide[J]. Chinese Journal of Luminescence, 2016,37(7): 881-886
姜利英, 周鹏磊, 肖小楠等. 基于氧化石墨烯荧光适体传感器的多巴胺检测[J]. 发光学报, 2016,37(7): 881-886 DOI： 10.3788/fgxb20163707.0881.

JIANG Li-ying, ZHOU Peng-lei, XIAO Xiao-nan etc. Fluorescent Aptamer Biosensor for The Detection of Dopamine with Graphene Oxide[J]. Chinese Journal of Luminescence, 2016,37(7): 881-886 DOI： 10.3788/fgxb20163707.0881.

摘要

以修饰有荧光基团(FAM)的多巴胺核酸适体作为识别元件

氧化石墨烯为猝灭剂

构建了光学适体传感器用于检测多巴胺。通过-堆积作用力

氧化石墨烯以共振方式把核酸适体上FAM能量转移到其表面

荧光信号消失;加入多巴胺后荧光恢复

荧光强度恢复的大小与多巴胺浓度呈正相关关系。实验优化结果表明

在反应时间5 min和10 g/mL氧化石墨烯条件下

氧化石墨烯可以达到对FAM的最高猝灭效率;25 min孵育后

多巴胺恢复荧光强度达到稳定;传感器线性检测范围为1~500 mol/L

检测限达到1 mol/L。所制备传感器具有检测范围宽、检测速度快、特异性强以及检测成本低等优点。

Abstract

A fluorescent aptamer biosensor was constructed for the determination of dopamine. The dopamine aptamer modified 6-carboxyfluorescein (FAM) was used as the recognition element

and graphene oxide was the quencher. Through - stacking interaction

FAM energy can be transferred to the surface of the graphene oxide in resonant manner

and the fluorescence disappears. In the presence of DA

the fluorescence can be recovered and the fluorescence intensity is found to be in proportion to the concentration of DA. The optimized research results show that 10g/mL graphene oxide may achieve maximum quenching efficiency within 5 min reaction time. The fluorescence intensity can reach a stable recovery after 25 min incubation. The response to dopamine proves to be linear with the correlation coefficient up to 0.988 in the relevant concentration range of 1-500 mol/L

and the detection limit is 1 mol/L. The biosensor is characterized by its wide range of measurement

fast detection

low cost and so on.

关键词

Keywords

references

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