Detection of DNA Based on Manganese-doped ZnS Quantum Dots/MTX Composite System

SI Fang-rui; MIAO Yan-ming; YANG Mao-qing; YAN Gui-qin

doi:10.3788/fgxb20163701.0013

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Detection of DNA Based on Manganese-doped ZnS Quantum Dots/MTX Composite System

更新时间：2020-08-12

- Detection of DNA Based on Manganese-doped ZnS Quantum Dots/MTX Composite System
- Chinese Journal of Luminescence Vol. 37, Issue 1, Pages: 13-21(2016)
- 作者机构：
  
  山西师范大学生命科学院,山西临汾,041000
- 作者简介：
- 基金信息：
- DOI：10.3788/fgxb20163701.0013
  CLC： O482.31
- Received：11 October 2015，
  
  Revised：13 November 2015，
  
  Published：10 January 2016
- 稿件说明：
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司芳瑞, 苗艳明, 杨茂青等. 基于MPA包覆的Mn掺杂ZnS量子点/米托蒽醌复合体系对DNA的检测[J]. 发光学报, 2016,37(1): 13-21

SI Fang-rui, MIAO Yan-ming, YANG Mao-qing etc. Detection of DNA Based on Manganese-doped ZnS Quantum Dots/MTX Composite System[J]. Chinese Journal of Luminescence, 2016,37(1): 13-21
司芳瑞, 苗艳明, 杨茂青等. 基于MPA包覆的Mn掺杂ZnS量子点/米托蒽醌复合体系对DNA的检测[J]. 发光学报, 2016,37(1): 13-21 DOI： 10.3788/fgxb20163701.0013.

SI Fang-rui, MIAO Yan-ming, YANG Mao-qing etc. Detection of DNA Based on Manganese-doped ZnS Quantum Dots/MTX Composite System[J]. Chinese Journal of Luminescence, 2016,37(1): 13-21 DOI： 10.3788/fgxb20163701.0013.

摘要

利用3-羟基丙酸(MPA)包覆的Mn掺杂ZnS量子点与盐酸米托蒽醌(MTX)构建了一种可简单测定DNA的磷光复合体系。该体系以MTX作为良好的电子受体

通过光诱导电子转移(PIET)原理猝灭Mn掺杂ZnS量子点的室温磷光(RTP)。当体系中加入DNA后

DNA通过静电和嵌插作用与MTX结合

形成更稳定的复合物

并使MTX从Mn掺杂ZnS量子点表面脱离

Mn掺杂ZnS量子点的RTP恢复

从而实现了对DNA的痕量检测。该体系检测DNA的线性范围为0.1~20 mgL

-1

检出限为0.07 mgL

-1

。该方法可有效避免其他共存物质的干扰并可应用于实际生物样品中DNA含量的快捷测定。

Abstract

A simple phosphorescent compound system for DNA detection was established by utilizing Mn (covered with 3-hydracrylicacid (MPA)) doped with ZnS quantum dots and mitoxantrone. By taking MTX as good electron acceptor

the room temperature phosphorescence (RTP) of Mn-doped ZnS quantum dots were quenched through photoinduced electron transfer principle. After the addition of DNA in system

DNA and MTX were combined through static electricity and intercalation

and a more stable compound was formed as well

which enable MTX removing from the surface of Mn-doped ZnS quantum dots and achieving RTP recovery of Mn-doped ZnS quantum dots. Thus

the trace detection of DNA was realized. The linear scope of this system for DNA detection and the detection limit is 0.1-20 mgL

-1

and 0.07 mgL

-1

respectively. This method can effectively avoid the interference from other coexisting substances

which can be used in rapid detection of DNA content in practical biological sample.

关键词

Keywords

references

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