Physical Characterization and Biological Toxicity of Chitosan-capped AgInS2 Quantum Dots

ZHANG Xiao-fan; LIU Li-wei; ZOU Peng; HU Si-yi; WANG Yue; ZHANG Xi-he

doi:10.3788/fgxb20153610.1118

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Physical Characterization and Biological Toxicity of Chitosan-capped AgInS₂ Quantum Dots

更新时间：2020-08-12

- Physical Characterization and Biological Toxicity of Chitosan-capped AgInS₂ Quantum Dots
- Chinese Journal of Luminescence Vol. 36, Issue 10, Pages: 1118-1125(2015)
- 作者机构：
  
  长春理工大学理学院,吉林长春,130022
- 作者简介：
- 基金信息：
- DOI：10.3788/fgxb20153610.1118
  CLC： O482.31
- Received：15 June 2015，
  
  Revised：12 August 2015，
  
  Published：10 October 2015
- 稿件说明：
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张晓凡, 刘丽炜, 邹鹏等. 壳聚糖包裹AgInS2的物理表征及生物毒性研究[J]. 发光学报, 2015,36(10): 1118-1125

ZHANG Xiao-fan, LIU Li-wei, ZOU Peng etc. Physical Characterization and Biological Toxicity of Chitosan-capped AgInS2 Quantum Dots[J]. Chinese Journal of Luminescence, 2015,36(10): 1118-1125
张晓凡, 刘丽炜, 邹鹏等. 壳聚糖包裹AgInS2的物理表征及生物毒性研究[J]. 发光学报, 2015,36(10): 1118-1125 DOI： 10.3788/fgxb20153610.1118.

ZHANG Xiao-fan, LIU Li-wei, ZOU Peng etc. Physical Characterization and Biological Toxicity of Chitosan-capped AgInS2 Quantum Dots[J]. Chinese Journal of Luminescence, 2015,36(10): 1118-1125 DOI： 10.3788/fgxb20153610.1118.

摘要

选用天然多糖中唯一的碱性多糖壳聚糖作为稳定剂和包裹剂

成功合成了水溶性的AgInS

量子点/低分子量壳聚糖纳米复合材料(AgInS

/LCSMS)。利用透射电子显微镜(TEM)、FT-IR傅里叶红外光谱仪、紫外吸收光谱、荧光分光光度计等表征手段对纳米复合材料的形貌、化学组成及光学性质进行了研究。结果表明

AgInS

/LCSMS纳米复合材料的粒径约为5~6 nm

在水相中仍具有较稳定的发光。之后

对AgInS

/LCSMS纳米复合材料的生物相容性进行了研究

对比AgInS

/LCSMS纳米复合材料与AgInS

量子点的细胞活性测试结果发现

AgInS

/LCSMS纳米复合材料的细胞活性比AgInS

量子点有了明显的提高

说明通过低分子量壳聚糖的包裹可以明显提高纳米材料的生物相容性。因此

这类具有较好水溶性和生物相容性的荧光 AgInS

/LCSMS纳米复合材料可作为优良的生物荧光标记材料在生物医学检验、细胞以及活体成像研究中有广泛的应用前景。

Abstract

The water-soluble AgInS

quantum dots/low molecular weight chitosan nanocomposites (AgInS

/LCSMS) were successfully synthesized. Natural polysaccharide in alkaline polysaccharideChitosan was used as stabilizer and capped agent. The structure

chemical composition

and optical properties of AgInS

/LCSMS nanocomposites were studied by transmission electron microscopy (TEM)

Fourier transform infrared spectroscopy (FT-IR)

fluorescence microscope

fluorescence spectrophotometer

and UV-Vis absorption spectroscopy

etc

. The results show that the particle diameter of AgInS

/LCSMS nanocomposites is about 5-6 nm

and they are still have a relatively stable luminescence in the water phase. In addition

we studied the biological compatibility of the AgInS

/LCSMS nanocomposites. Compared with pure AgInS

quantum dots

the cell activity of AgInS

/LCSMS nanocomposites has an obvious improvement. It shows that low molecular weight chitosan wrapping can obviously improve the biocompatibility of the nanometer materials. Therefore

this kind of AgInS

/LCSMS fluorescent nanocomposites with good water solubility and biological compatibility show significant value for potential applications in biomedicine test

biolabel

cell and

in vivo

imaging studies.

关键词

Keywords

references

Zhong H, Bai Z, Zou B. Tuning the luminescence properties of colloidal Ⅰ-Ⅲ-Ⅵ semiconductor nanocrystals for optoelectronics and biotechnology applications [J]. J. Phys. Chem. Lett., 2012, 21(3):3167-3175.

Wang S. The preparation of chitosan microspheres [J]. Technol. Equip.(工艺与设备), 2006(8):13-14 (in Chinese).

Ding X B, Sun Z H, Wan G X. Heat sensitive polymer package and synthesis of magnetic chitosan [J]. J. Polym.(高分子学报), 1998(5):628-631 (in Chinese).

Jiang W, Li F S, Yang Y, et al. The preparation of magnetic chitosan composite microspheres and performance research [J]. J. Mater. Sci. Eng.(材料科学与工程学报), 2004, 22(5):660-662 (in Chinese).

Park J H, Im K H, Lee S H, et al. Preparation and characterization of magnetic chitosan particles for hyperthermia application [J]. J. Magn. Magnet. Mater., 2005, 293:328-333

Zhang H Z, Sun X M , Li B H. The preparation of magnetic chitosan microsphere and its adsorption on abscisic acid [J]. J. Wuhan Univ.(武汉大学学报), 2007, 53(6):669-673 (in Chinese).

Zhang C, Qu G, Sun Y, et al. Biological evaluation of N-octyl-O-sulfate chitosan as a new nano-carrier of intravenous drugs [J]. Eur. J. Pharm. Sci., 2008, 33(4-5):415-423.

Grenha A, Remunn-Lpez C, Carvalho E L S, et al. Microspheres containing lipid/chitosan nanoparticles complexes for pulmonary delivery of therapeutic proteins [J]. Eur. J. Pharm. Biopharm., 2008, 69(1):83-93.

Sinha V R, Singla A K, Wadhawan S, et al. Chitosan microspheres as a potential carrier for drugs [J]. Int. J. Pharm., 2004, 274(1-2):1-33.

Pedro A S, Cabral-Albuquerque E, Ferreira D, et al. Chitosan: An option for development of essential oil delivery systems for oral cavity care?[J]. Carbohyd. Polym., 2009, 76(4):501-508.

Genta I, Costantini M, Asti A, et al. Influence of glutaraldehyde on drug release and mucoadhesive properties of chitosan microspheres [J]. Carbohyd. Polym., 1998, 36(2):81-88.

Lorenzo-Lamosa M L, Remunn-Lpez C, Vila-Jato J L, et al. Design of microencapsulated chitosan microspheres for colonic drug delivery [J]. J. Control. Release, 1998, 52(1-2):109-118.

Gu Y H, Wang L, Tan T W, et al. Preparation of uniform-sized chitosan microspheres and application as carriers for protein drugs [J]. Chin. J. Biotechnol.(生物工程学报), 2006, 22(1):150-155(in Chinese).

Taqieddin E, Amiji M. Enzyme immobilization in novel alginate-chitosan core-shell microcapsules [J]. Biomaterials, 2004, 25(10):1937-45.

El-Hameed M D A, Kellaway I W. Preparation and in vitro characterisation of mucoadhesive polymeric microspheres as intra-nasal delivery systems [J]. Eur. J. Pharm. Biopharm., 1997, 44(1):53-60.

Shirasaki Y, Supran G J, Bawendi M G, et al. Emergence of colloidal quantum-dot light-emitting technologies [J]. Nat. Photon., 2013, 7(1):13-23.

Zhao J Y. The Preparation of Chitosan Nano Fluorescent Probes, Characterization and Application [D]. Hangzhou: Zhejiang University, 2006 (in Chinese).

Tuan V D. Biomedical Photonics Handbook [M]. Florida: CRC Press, 2003:921.

Qu J L. Theory and Experimental Research of Fluorescence Lifetime Imaging Microscopy[D]. Xi'an: Xi'an Institute of Optics and Precision Mechanics, Chinese Academy of Sciences, 1998 (in Chinese).

Periasamy A, Sharman K K, Ahuja R, et al. A single green fluorescent protein fluorescence lifetime imaging in living cells [J]. SPIE, 1999, 3604:6212-6218.

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