Folic Acid-conjugated AgInS2 Quantum Dots for in vitro Cancer Cell Imaging

BU Cheng-fei; LIU Li-wei; WANG Qian; ZHANG Bu-tian; HU Si-yi; REN Yu; ZHU Ling-xi

doi:10.3788/fgxb20153609.0989

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Folic Acid-conjugated AgInS₂ Quantum Dots for in vitro Cancer Cell Imaging

更新时间：2020-08-12

- Folic Acid-conjugated AgInS₂ Quantum Dots for in vitro Cancer Cell Imaging
- Chinese Journal of Luminescence Vol. 36, Issue 9, Pages: 989-995(2015)
- 作者机构：
  
  1. 长春理工大学理学院国际纳米光子学与生物光子学联合研究中心,吉林长春,130022
  2. 南洋理工大学电气与电子工程学院,新加坡
- 作者简介：
- 基金信息：
- DOI：10.3788/fgxb20153609.0989
  CLC： O482.31
- Received：14 June 2015，
  
  Revised：24 July 2015，
  
  Published：03 September 2015
- 稿件说明：
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卜承飞, 刘丽炜, 王摇倩等. 叶酸修饰的AgInS2量子点在体外癌细胞成像上的应用[J]. 发光学报, 2015,36(9): 989-995

BU Cheng-fei, LIU Li-wei, WANG Qian etc. Folic Acid-conjugated AgInS2 Quantum Dots for in vitro Cancer Cell Imaging[J]. Chinese Journal of Luminescence, 2015,36(9): 989-995
卜承飞, 刘丽炜, 王摇倩等. 叶酸修饰的AgInS2量子点在体外癌细胞成像上的应用[J]. 发光学报, 2015,36(9): 989-995 DOI： 10.3788/fgxb20153609.0989.

BU Cheng-fei, LIU Li-wei, WANG Qian etc. Folic Acid-conjugated AgInS2 Quantum Dots for in vitro Cancer Cell Imaging[J]. Chinese Journal of Luminescence, 2015,36(9): 989-995 DOI： 10.3788/fgxb20153609.0989.

摘要

成功制备出高品质的三元AgInS

量子点。通过配体交换法将油溶性AgInS

量子点转为水溶性量子点

通过dBSA修饰水溶性量子点形成配位体壳

使量子点具有更好的稳定性(4周)。从透射电子显微镜(TEM)观察到dBSA修饰后的量子点的粒径增加

分散性较好

并且在可见光区域有明显的光致发光。用叶酸对dBSA-MPA量子点进行修饰

并通过傅立叶变换红外光谱进行了验证。将得到的FA-dBSA-MPA纳米复合材料应用于能与叶酸受体特异性结合的乳腺癌细胞中

并在荧光倒置显微镜中检测到量子点成功对乳腺癌细胞进行了标记。与dBSA-MPA量子点相比

表面被叶酸修饰后的量子点与癌细胞的结合效率显著提高。

Abstract

AgInS

quantum dots (QDs) were synthesized using hot colloidal method and transferred into water via a ligand exchange route. To enhance the stability of these QDs

dBSA was coated on the aqueous QDs as a ligand shell. After dBSA modification

a size increase was observed from the transmission electron microscopy (TEM) results. The prepared dBSA-MPA QDs exhibited good monodispersity

enhanced stability (stable for 4 weeks) and bright photoluminescence. Then the QDs were functionalized with folic acid (FA) and the successful conjugation was confirmed by the Fourier transform infrared (FT-IR) spectroscopy. The resulting FA-dBSA-MPA QDs nanocomposites were tested in breast cancer cells (MCF-7 cells) with elevated folate receptor expression. Compared to the unconju-gated dBSA-MPA QDs

the presence of FA on the surface of QDs significantly improved the uptake rate of the nanop-articles by the cancer cells.

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