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1. 吉林大学 仪器科学与电气工程学院,吉林 长春,130021
2. 中检国研(北京)科技有限公司 北京,100123
3. 中国检验检疫科学研究院 北京,100123
Received:23 April 2015,
Revised:13 May 2015,
Published:03 July 2015
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陈文洋, 邹明强, 刘峰等. 化学发光检测光子计数的统计特性[J]. 发光学报, 2015,36(7): 854-859
CHEN Wen-yang, ZOU Ming-qiang, LIU Feng etc. Photon Counting Statistical Analysis for Chemiluminescence Detection[J]. Chinese Journal of Luminescence, 2015,36(7): 854-859
陈文洋, 邹明强, 刘峰等. 化学发光检测光子计数的统计特性[J]. 发光学报, 2015,36(7): 854-859 DOI: 10.3788/fgxb20153607.0854.
CHEN Wen-yang, ZOU Ming-qiang, LIU Feng etc. Photon Counting Statistical Analysis for Chemiluminescence Detection[J]. Chinese Journal of Luminescence, 2015,36(7): 854-859 DOI: 10.3788/fgxb20153607.0854.
对于增强化学发光酶联免疫分析而言
检测结果、计数效率、信噪比等重要参数不仅与光子计数装置有关
而且与光学发光体系的光子脉冲发射统计过程相关。本文实验测量了增强鲁米诺化学发光体系的光子脉冲的统计特性
并根据其脉冲发射时间统计特性对化学发光光子计数检测装置的设计进行了理论分析。研究结果表明
化学发光的光子脉冲发射间隔呈Weibull分布。
In enhanced chemiluminescence enzyme-linked immunoassay
major parameters such as detection result
efficiency of counting and
S/N
ratio
are related with not only photon counting devices but also statistics process of photon pulse emission in optical luminescence system. In this article
we measured the statistics characteristics of photon pulse in enhanced luminol chemiluminescence system and theoretically analyzed the design of photon counting detection device of chemiluminescence system by its statistics characteristics of its pulse emission time. The research suggests that the photon emission pulse intervals of chemiluminescence subject to Weibull distribution.
Wang J. Study on Immunoassay for Fumonisin B1[D]. Shanghai: Shanghai Jiao Tong University, 2010 (in Chinese).
Zhou Y, Zhou T, Zhou R, et al. Chemiluminescence immunoassay for the rapid and sensitive detection of antibody against porcine parvovirus by using horseradish peroxidase/detection antibody-coated gold nanoparticles as nanoprobes [J]. J. Lumin., 2014, 29(4):338-343.
Fan F, Shen H Y, Zhang G J, et al. Chemiluminescence immunoassay based on microfluidic chips for -fetoprotein [J]. Clin. Chim. Acta, 2014, 431:113-117.
Ma L, Sun Y Y, Kang X J, et al. Chemiluminescence development of nanobody-based flow injection chemiluminescence immunoassay for sensitive detection of human prealbumin [J]. Biosens. Bioelectron., 2014, 61:165-171.
Zhang H S, Qi S W. A rapid and sensitive chemiluminescence immunoassay based on magnetic particles for squamous cell carcinoma antigen in human serum [J]. Clin. Chim. Acta, 2011, 412(17):1572-1577.
Ma X X, Liu H Z, Tang J J, et al. Comparison of three enzyme-linked immunosorbent assay methods for quantitative determination of ricin [J]. Chin. J. Chem.(分析化学), 2011, 39(5):685-689 (in Chinese).
Zhang J R, Chen J, Liu Z M. Progress of electrochemical luminescence immunoassay technology [J]. Chin. J. Chem.(分析化学), 2010, 38(8):1219-1226 (in Chinese).
Zheng G J, Fang L Q, Chen H, et al. Magnetic microparticle chemiluminescence immunoassay method for determination of estriol in human urine [J]. Chin. J. Chem.(分析化学), 2011, 39(1):62-66 (in Chinese).
Xue P, Zhang Z J, Zhang X X. Chemiluminescent analysis of carcinoembryonic antigen in serum samples [J]. Chin. J. Chem.(分析化学), 2011, 39(1):95-98 (in Chinese).
Shu L T, Xu B S, Zhang P Y. Enhanced chemiluminescence enzyme immunoassay for determination of serum gastrin and establishment [J]. Chin. J. Lab. Diagn.(中国实验诊断学), 2006, 10(1):96-97 (in Chinese).
Chong K, Gui J, Tan K H. Enhanced chemiluminescence(ECL) for gene detection [J]. Plant Physiol. Commun.(植物生理学通讯), 1993, 29(5):368-370 (in Chinese).
Wang Y, Zhu H Q, Chen H Z, et al. Protein imprinting technology [J]. Acta Univ. Med. Anhui (安徽医科大学学报), 2004, 39(3):245-247 (in Chinese).
Wang C, Wu J, Zong C, et al. Chemiluminescent immunoassay and its application [J]. Chin. J. Chem.(分析化学), 2012, 40(1):3-10 (in Chinese).
Kricka L J. Chemiluminescence and bioluminescence analysis [J]. Ency. Mol. Biol. Moi. Med., 1996, 1:322-332.
Lin J M. Chemiluminescence Basic Theory and Application [M]. Beijing: Chemical Industry Press, 2004 (in Chinese).
Zhao L X. Research and Application of The Chemiluminescence Enzyme-linked Immunoassay[D]. Beijing: Research Center for Eco-environmental Sciences, 2005 (in Chinese).
Zhu Y, Ouyang J. Design and implementation of the single photon counter system [J]. Chin. J. Sci. Instrum.(仪器仪表学报), 2007, 28(4):29-30 (in Chinese).
Liu G F, Xiong K W, Liu W C. Study on the single photon counter system [J]. J. Sci. Teachers' College Univ.(高师理科学报), 2011, 31(2):67-70 (in Chinese).
Shao Y J, Zhu L Q, Guo Y K, et al. Single photon counting system and its noise analysis [J]. Modern Electron. Tech.(现代电子技术), 2013, 36(6):168-170 (in Chinese).
Zhao J P, Zhong Y X. Determination of single photon counting system for discriminating voltage [J]. Digital Technol. Appl.(数字计数与应用), 2012, 11:53-54 (in Chinese).
Wang T F. Method of improving the detectability of photon counting system based on PMT [J]. Opt. Electric. Inform.(光机电信息), 2009, 26(3):37-42 (in Chinese).
Ma F S, Zhou W, Liu C B. The Six Sigma Management Statistical Guide to UsingMINITAB Guidance [M]. Beijing: China Renmin University Press, 2007 (in Chinese).
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