Comparative Studies on The Reaction Mechanism of Pioglitazone Hydrochloride with Bovine Serum Albumin by Fluorescence Spectroscopy and Synchronous Fluorescence Spectroscopy

LI Gai-xia; LIU Bao-sheng; LI Zhi-yun; FU Jia-he; WANG Tian-cheng

doi:10.3788/fgxb20153606.0705

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Comparative Studies on The Reaction Mechanism of Pioglitazone Hydrochloride with Bovine Serum Albumin by Fluorescence Spectroscopy and Synchronous Fluorescence Spectroscopy

更新时间：2020-08-12

- Comparative Studies on The Reaction Mechanism of Pioglitazone Hydrochloride with Bovine Serum Albumin by Fluorescence Spectroscopy and Synchronous Fluorescence Spectroscopy
- Chinese Journal of Luminescence Vol. 36, Issue 6, Pages: 705-710(2015)
- 作者机构：
  
  河北大学化学与环境科学学院河北省分析科学技术重点实验室,河北保定,071002
- 作者简介：
- 基金信息：
- DOI：10.3788/fgxb20153606.0705
  CLC： O657.3
- Received：27 March 2015，
  
  Revised：22 April 2015，
  
  Published：03 June 2015
- 稿件说明：
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李改霞, 刘保生, 李志云等. 盐酸吡格列酮与BSA反应机理的荧光猝灭法、同步荧光法比较研究[J]. 发光学报, 2015,36(6): 705-710

LI Gai-xia, LIU Bao-sheng, LI Zhi-yun etc. Comparative Studies on The Reaction Mechanism of Pioglitazone Hydrochloride with Bovine Serum Albumin by Fluorescence Spectroscopy and Synchronous Fluorescence Spectroscopy[J]. Chinese Journal of Luminescence, 2015,36(6): 705-710
李改霞, 刘保生, 李志云等. 盐酸吡格列酮与BSA反应机理的荧光猝灭法、同步荧光法比较研究[J]. 发光学报, 2015,36(6): 705-710 DOI： 10.3788/fgxb20153606.0705.

LI Gai-xia, LIU Bao-sheng, LI Zhi-yun etc. Comparative Studies on The Reaction Mechanism of Pioglitazone Hydrochloride with Bovine Serum Albumin by Fluorescence Spectroscopy and Synchronous Fluorescence Spectroscopy[J]. Chinese Journal of Luminescence, 2015,36(6): 705-710 DOI： 10.3788/fgxb20153606.0705.

摘要

利用荧光猝灭法和同步荧光法进行了盐酸吡格列酮(PGH)与牛血清白蛋白(BSA)之间反应机理的比较研究。结果表明:两种方法在研究PGH-BSA体系的猝灭类型、结合常数、结合位置、作用力类型、协同性及能量转移距离等方面

得到了相同的结果

表明同步荧光光谱法可以用于药物与蛋白反应机理的研究。

Abstract

The reaction mechanism between pioglitazone hydrochloride (PGH) and bovine serum albumin (BSA) was studied by fluorescence quenching method and synchronous fluorescence method under different temperatures. The results show that the conclusions of two methods are identical on the quenching mechanism

type of interaction force

binding constants

binding location

synergistic effect

energy-transfer parameters of pioglitazone hydrochloride with bovine serum albumin

etc

. These indicate that the synchronous fluorescence spectroscopy can be used to replace the traditional fluorescence spectroscopy to study the reaction mechanism of drugs with protein.

关键词

Keywords

references

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