Comparative Study of Binding Constants Between Small Molecule Drugs and Protein Calculated by Different Equation

GUO Ying; LIU Bao-sheng; LI Zhi-yun; ZHANG Li-hui; LYU Yun-kai

doi:10.3788/fgxb20143509.1114

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Comparative Study of Binding Constants Between Small Molecule Drugs and Protein Calculated by Different Equation

更新时间：2020-08-12

- Comparative Study of Binding Constants Between Small Molecule Drugs and Protein Calculated by Different Equation
- Chinese Journal of Luminescence Vol. 35, Issue 9, Pages: 1114-1119(2014)
- 作者机构：
  
  河北大学化学与环境科学学院,河北保定,071002
- 作者简介：
- 基金信息：
- DOI：10.3788/fgxb20143509.1114
  CLC： O658.9
- Received：13 May 2014，
  
  Revised：03 June 2014，
  
  Published Online：27 June 2014，
  
  Published：03 September 2014
- 稿件说明：
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郭莹, 刘保生, 李志云等. 不同方程计算药物小分子与蛋白结合常数的比较研究[J]. 发光学报, 2014,35(9): 1114-1119

GUO Ying, LIU Bao-sheng, LI Zhi-yun etc. Comparative Study of Binding Constants Between Small Molecule Drugs and Protein Calculated by Different Equation[J]. Chinese Journal of Luminescence, 2014,35(9): 1114-1119
郭莹, 刘保生, 李志云等. 不同方程计算药物小分子与蛋白结合常数的比较研究[J]. 发光学报, 2014,35(9): 1114-1119 DOI： 10.3788/fgxb20143509.1114.

GUO Ying, LIU Bao-sheng, LI Zhi-yun etc. Comparative Study of Binding Constants Between Small Molecule Drugs and Protein Calculated by Different Equation[J]. Chinese Journal of Luminescence, 2014,35(9): 1114-1119 DOI： 10.3788/fgxb20143509.1114.

摘要

在Tris-HCl缓冲溶液（pH=7.4）中，分别研究了莫西沙星、诺氟沙星、氧氟沙星、恩诺沙星、洛美沙星、环丙沙星与牛血清白蛋白的相互作用，利用计算药物小分子与蛋白结合常数的常用方程：Scatchard修正方程、Lineweaver-Burk双倒数方程、双对数方程、修正的Stern-Volmer方程、改进的双对数方程，分别计算了各沙星药物与蛋白的结合常数和结合位点数。对计算结果进行比较，结果表明对于药物与蛋白为1：1的结合反应，除Scatchard修正方程外其他方程的计算结果十分接近，其中改进的双对数方程的计算结果更加合理准确。

Abstract

In Tris-HCl buffer solution (pH=7.4)

the interactions of moxifloxacin

norfloxacin

ofloxacin

enrofloxacin

lomefloxacin

and ciprofloxacin with bovine serum albumin were studied

respectively. Using modified Scatchard equation

Lineweaver-Burk double reciprocal equation

double logarithmic equation

modified Stern-Volmer equation

and improved double logarithmic equation

the binding constants and binding points of drugs with bovine serum albumin were calculated

respectively. For the binding reaction of drug-protein ratio of 1:1

the calculation results are very close except modified Scatchard equation

and the improved double logarithmic equation is the most accurate.

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Keywords

references

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