Determination of Terazosin Hydrochloride by Using Bovine Serum Albumin as Fluorescent Probe

JI Ren-dong; ZHAO Zhi-min; ZHANG Ji-hua; CHEN Meng-lan; ZHU Xing-yue

doi:10.3788/fgxb20143508.1014

您当前的位置：

首页 >

文章列表页 >

Determination of Terazosin Hydrochloride by Using Bovine Serum Albumin as Fluorescent Probe

更新时间：2020-08-12

- Determination of Terazosin Hydrochloride by Using Bovine Serum Albumin as Fluorescent Probe
- Chinese Journal of Luminescence Vol. 35, Issue 8, Pages: 1014-1017(2014)
- 作者机构：
  
  1. 南京航空航天大学理学院,江苏南京,210016
  2. 淮阴工学院电子与电气工程学院,江苏淮安,223003
- 作者简介：
- 基金信息：
- DOI：10.3788/fgxb20143508.1014
  CLC： O433.4
- Received：13 May 2014，
  
  Revised：03 June 2014，
  
  Published：03 August 2014
- 稿件说明：
移动端阅览
季仁东, 赵志敏, 张吉华等. 基于牛血清白蛋白荧光探针的盐酸特拉唑嗪含量测定[J]. 发光学报, 2014,35(8): 1014-1017

JI Ren-dong, ZHAO Zhi-min, ZHANG Ji-hua etc. Determination of Terazosin Hydrochloride by Using Bovine Serum Albumin as Fluorescent Probe[J]. Chinese Journal of Luminescence, 2014,35(8): 1014-1017
季仁东, 赵志敏, 张吉华等. 基于牛血清白蛋白荧光探针的盐酸特拉唑嗪含量测定[J]. 发光学报, 2014,35(8): 1014-1017 DOI： 10.3788/fgxb20143508.1014.

JI Ren-dong, ZHAO Zhi-min, ZHANG Ji-hua etc. Determination of Terazosin Hydrochloride by Using Bovine Serum Albumin as Fluorescent Probe[J]. Chinese Journal of Luminescence, 2014,35(8): 1014-1017 DOI： 10.3788/fgxb20143508.1014.

摘要

对牛血清白蛋白（BSA）与盐酸特拉唑嗪（THD）混合体系进行了荧光发射光谱和同步光谱实验研究。BSA溶液在340 nm处有明显荧光特征峰，当加入THD后导致牛血清白蛋白发生内源荧光猝灭。据此建立了以BSA为探针的THD含量测定方法，通过该方法所得的模型函数相关系数都高于0.99。对所得模型函数进行了实验验证，其中发射光谱模型函数回收率为96.04%~103.16%，同步光谱模型函数回收率为100.41%~ 105.58%，相对标准偏差分别为3.70%和2.42%。荧光发射光谱和同步光谱方法的检出限分别为0.6410

-8

mol/L和0.6610

-8

mol/L，定量限分别为0.2110

-7

mol/L和0.2210

-7

mol/L。

Abstract

The fluorescence emission and synchronous spectra of the bovine serum albumin (BSA) and terazosin hydrochloride (THD) mixed system were studied. The most intensive characteristic peak (340 nm) was found in the spectrum of BSA solution

and the addition of THD resulted in the fluorescence quenching of BSA. Based on this phenomenon

the determination method for THD was established

in which BSA was used as the fluorescent probe. The correlation coefficients of the model functions are all more than 0.99. The recoveries of emission and synchronous spectra are 96.04%~103.16% and 100.41%~105.58%

the relative standard deviations (RSD) are 3.70% and 2.42%

the limits of detection are 0.6410

-8

mol/L and 0.6610

-8

mol/L

and the limits of quantification are 0.2110

-7

mol/L and 0.2210

-7

mol/L

respectively.

关键词

Keywords

references

Li J H. Synthesis of terazosin [J]. Chin. J. Pharmaceut.(中国医药工业杂志), 1998, 29(4):147-149 (in Chinese).

Ma T S, Li G, Song S C, et al. Study on preparation and delivery characteristics of terazosin hydrochloride osmotic pump controlled release tablets [J]. China Pharmaceut.(中国药业), 2010, 19(13):51-53 (in Chinese).

Zou H Y, Wu H L, OuYang L Q, et al. Fluorescent quantification of terazosin hydrochloride content in human plasma and tablets using second-order calibration based on both parallel factor analysis and alternating penalty trilinear decomposition [J]. Anal. Chim. Acta, 2009, 650(1):143-149.

Wang S Q. Determination of terazosin hydrochloride in terazosin hydrochloride tables by HPLC [J]. Chem. Eng.(化学工程师), 2006, 133(10):34-35 (in Chinese).

Sekhar E C, Rao T R K, Sekhar K R, et al. Determination of terazosin in human plasma, using highperformance liquid chromatography with fluorescence detection [J]. J. Chromatogr. B, 1998, 710(1-2):137-142.

Liu K J, Li S D. Determination of terazosin hydrochloride capsules by fluorescence spectroscopy [J]. Chin. J. Modern Appl. Pharmacy (中国现代应用药学), 2005, 22(6):487-489 (in Chinese).

Silva1 R A, Masi A N. Sensitive determination of terazosin by X-ray fluorescence spectrometry based on the formation of ion-pair associates with zinc thiocyanate [J]. X-Ray Spectrometry, 2007, 36(4):279-283.

Ragaa E S, Nahla S E, Gouda Ayman A, et al. Extractive spectrophotometric determination of some -adrenergic-antagonists in pure forms and in pharmaceutical formulations [J]. Chem. Ind. Chem. Eng. Q, 2012, 18(2):179-191.

Abdinea H H, El-Yazbia F A, Blaiha S M, et al. Spectrophotometric and spectrofluorimetric methods for the determination of terazosin in dosage forms [J]. Spectrosc. Lett., 1998, 31(5):969-980.

Cao S N, Liu B S, Li Z Y, et al. A determination method of glipizide content with bovine serum albumin as fluorescent probe [J]. Chin. J. Lumin.(发光学报), 2013, 34(7):930-934 (in Chinese).

Ji R D, Zhao Z M, Zhang L, et al. Study on experiment of fluorescence spectra detection of pesticide residue about imidacloprid in apple juices [J]. Spectrosc. Spect. Anal.(光谱学与光谱分析), 2013, 33(3):668-671 (in Chinese).

Poulli K I, Mousdis G A, Georgiou C A. Synchronous fluorescence spectroscopy for quantitative determination of virgin olive oil adulteration with sunflower oil [J]. Anal. Bioanal. Chem., 2006, 386(5):1571-1575.

Wang T H, Zhao Z M, Wei B Z, et al. Spectroscopic investigations on the binding of dibazol to bovine serum albumin [J]. J. Mol. Struct., 2010, 970(1-3):128-133.

Views

177

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Interaction Between Progesterone and Bovine Serum Albumin by Fluorescence Spectrum and Molecular Docking

Interaction of Rare Earth with Bovine Serum Albumin

The Preliminary Analysis on the Fluorescence Spectra of Bilirubin in Human Serum

Deteramination of Procymidone Residues Content in Honey by Fluorescence Spectroscopy

Investigation on The Interaction between p-Coumaric Acid and Human Serum Albumin by Fluorescence and Surface Enhanced Raman Spectra

Related Author

YANG Shu-ling

LIAO Xian-ping

FAN Xing

TUO Xun

FENG Xiao-qiang

FU Guo-qing

LI Xiao-fang

YANG Sheng

Related Institution

School of Pharmacy, Nanchang University

College of Chemistry, Nanchang University

College of Life Science and Chemistry Tianshui Normal University

Tianshui Traditional Chinese Medical Hospital

Chemistry and Chemical Engineering College, Lanzhou University

AI问答

Address：No.3888 Dong Nanhu Road, Changchun, Jilin, China Postal code：130033
Tel：0431-86176862 Email：fgxbt@126.com
Technical support is provided by Beijing Founder electronics co., LTD 吉ICP备11002662号-17 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰