WEI Feng, LI Xin, WANG Ai-ling etc. Synthesis, Spectral Studies on The Interaction of DNA and Rare Earth with Salicylideneglycine Schiff and Nitrogen-heterocyclic Complexes[J]. Chinese Journal of Luminescence, 2014,35(3): 366-371
WEI Feng, LI Xin, WANG Ai-ling etc. Synthesis, Spectral Studies on The Interaction of DNA and Rare Earth with Salicylideneglycine Schiff and Nitrogen-heterocyclic Complexes[J]. Chinese Journal of Luminescence, 2014,35(3): 366-371 DOI: 10.3788/fgxb20143503.0366.
Synthesis, Spectral Studies on The Interaction of DNA and Rare Earth with Salicylideneglycine Schiff and Nitrogen-heterocyclic Complexes
A series of novel rare earth complexes were synthesized with salicylideneglycine schiff base (Sal-GlyK)
l
10-phenanthroline (Phen) and 2
2'-bipyridine (Bipy). The complexes were characterized by elemental analysis
rare earth coordination titration
molar conductivity measurement
IR spectroscopy and UV-Vis absorption spectroscopy. They were confirmed to be
RE
(Sal-Gly)(NO
3
)2H
2
O
RE
(Sal-Gly)(Phen)(NO
3
)H
2
O and
RE
(Sal-Gly)(Bipy)(NO
3
)H
2
O (
RE
3+
=Eu
3+
La
3+
) as the results of those measurement. The interaction between the rare earth complexes and calf thymus DNA (CT-DNA) was investigated by UV-Vis spectrophotometry and fluorescence spectroscopy. Red shift and hypochromicity of the absorption peak from UV spectra of the complexes were founded in the presence of DNA
and fluorescence quench experiment was used to confirm that the complexes can quench the fluorescence of ethidium bromide-DNA (EB-DNA) system. All these results indicate that intercalation model exists between the complexes and DNA
and good planarity and large surface area of the ligand have a positive effect on the intercalative reaction
so the sequence of binding ability of the complexes to DNA is
RE
(Sal-Gly)(Phen)(NO
3
)H
2
O
>
RE
(Sal-Gly)(Bipy)(NO
3
)H
2
O
>
RE
(Sal-Gly)(NO
3
)2H
2
O.
关键词
Keywords
references
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