Interaction of Succinic-oligochitosan Rare Earth Complexes with Herring Sperm DNA by Electrochemical and Spectral Methods

LI Xiao-fang; FENG Xiao-qiang; YANG Sheng; WANG Xia

doi:10.3788/fgxb20133412.1662

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Interaction of Succinic-oligochitosan Rare Earth Complexes with Herring Sperm DNA by Electrochemical and Spectral Methods

更新时间：2020-08-12

- Interaction of Succinic-oligochitosan Rare Earth Complexes with Herring Sperm DNA by Electrochemical and Spectral Methods
- Chinese Journal of Luminescence Vol. 34, Issue 12, Pages: 1662-1666(2013)
- 作者机构：
  
  1. 天水师范学院生命科学与化学学院,甘肃天水,74100
  2. 定西师范高等专科学校,甘肃定西,743000
- 作者简介：
- 基金信息：
- DOI：10.3788/fgxb20133412.1662
  CLC： O614.121
- Received：26 July 2013，
  
  Revised：25 September 2013，
  
  Published：10 December 2013
- 稿件说明：
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李小芳, 冯小强, 杨声, 王霞. 丁二酰化壳寡糖稀土配合物与鲱鱼精DNA相互作用的电化学及光谱学研究[J]. 发光学报, 2013,34(12): 1662-1666

LI Xiao-fang, FENG Xiao-qiang, YANG Sheng, WANG Xia. Interaction of Succinic-oligochitosan Rare Earth Complexes with Herring Sperm DNA by Electrochemical and Spectral Methods[J]. Chinese Journal of Luminescence, 2013,34(12): 1662-1666
李小芳, 冯小强, 杨声, 王霞. 丁二酰化壳寡糖稀土配合物与鲱鱼精DNA相互作用的电化学及光谱学研究[J]. 发光学报, 2013,34(12): 1662-1666 DOI： 10.3788/fgxb20133412.1662.

LI Xiao-fang, FENG Xiao-qiang, YANG Sheng, WANG Xia. Interaction of Succinic-oligochitosan Rare Earth Complexes with Herring Sperm DNA by Electrochemical and Spectral Methods[J]. Chinese Journal of Luminescence, 2013,34(12): 1662-1666 DOI： 10.3788/fgxb20133412.1662.

摘要

采用紫外光谱和循环伏安法，研究了丁二酰化壳寡糖稀土配合物（BCS-La、BCS-Nd）与鲱鱼精DNA之间的作用方式。BCS-La、BCS-Nd的存在导致Fe（CN）

3-/4-

探针分子峰电流下降，式量电位正移，表明BCS-La、BCS-Nd和探针分子与DNA之间存在竞争性作用；BCS-La和BCS-Nd分子都是通过插入方式与DNA相互作用。在一定的扫描速率范围内（0.01~0.2 V/s），在BCS-La或BCS-Nd参与的条件下，Fe（CN）

3-/4-

在Au/DNA电极上的反应受吸附控制。BCS-La和BCS-Nd分别使得鲱鱼精DNA的特征峰产生明显的减色效应，最大吸收峰峰位红移，进一步表明BCS-La和BCS-Nd分别以插入方式与鲱鱼精DNA发生相互作用，导致DNA分子的构象变化。BCS-La与DNA的结合比为：

（BCS-La）：

（DNA）=2：1；

（BCS-Nd）：

（DNA）=6：1。

Abstract

The interaction between succinic-oligochitosan-rare earth complexes (BCS-La and BCS-Nd) and herring sperm DNA was investigated by cyclic voltammetry and UV-Vis spectra. The results indicated as follows. Firstly

the peak current of probe molecule Fe(CN)

3-/4-

at Au/DNA electrodes obviously decreased and the peak potential shifted positively because of the existences of complexes. All above these showed that there was a competitive effect between complexes and probe molecule with herring sperm DNA

which revealed that a binding mode intercalation was interacted between complexes and herring sperm DNA. The reaction of Fe(CN)

3-/4-

at Au/DNA electrodes was controlled by adsorption mechanism in the range of scan rate from 0.01 V/s to 0.2 V/s. Secondly

the absorption intensity of herring sperm DNA decreased with the adding of complexes

and the maximum absorption peak had a red shift

which further illustrated that a binding mode intercalation was interacted between complexes and herring sperm DNA

thus led to the change of DNA conformation. The combining ratio of

(BCS-La) and

(BCS-Nd) with DNA was 2:1 and 6:1

respectively.

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references

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