A New Use of 7-(diethylamino)-3-(pyridin-2-yl)coumarin： As Lysosome Fluorescence Probe

XIE Li-juan; LI Jian-min

doi:10.3788/fgxb20133411.1538

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A New Use of 7-(diethylamino)-3-(pyridin-2-yl)coumarin： As Lysosome Fluorescence Probe

更新时间：2020-08-12

- A New Use of 7-(diethylamino)-3-(pyridin-2-yl)coumarin： As Lysosome Fluorescence Probe
- Chinese Journal of Luminescence Vol. 34, Issue 11, Pages: 1538-1543(2013)
- 作者机构：
  
  1. 华侨大学生物医学学院,福建泉州,362021
  2. 华侨大学分子药物教育部工程研究中心,福建泉州,362021
- 作者简介：
- 基金信息：
- DOI：10.3788/fgxb20133411.1538
  CLC： O657.3
- Received：21 June 2013，
  
  Revised：17 August 2013，
  
  Published：10 November 2013
- 稿件说明：
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解丽娟, 李建民. 7-二乙胺基-3-(2'-吡啶)香豆素的新用途：溶酶体荧光探针[J]. 发光学报, 2013,34(11): 1538-1543

XIE Li-juan, LI Jian-min. A New Use of 7-(diethylamino)-3-(pyridin-2-yl)coumarin： As Lysosome Fluorescence Probe[J]. Chinese Journal of Luminescence, 2013,34(11): 1538-1543
解丽娟, 李建民. 7-二乙胺基-3-(2'-吡啶)香豆素的新用途：溶酶体荧光探针[J]. 发光学报, 2013,34(11): 1538-1543 DOI： 10.3788/fgxb20133411.1538.

XIE Li-juan, LI Jian-min. A New Use of 7-(diethylamino)-3-(pyridin-2-yl)coumarin： As Lysosome Fluorescence Probe[J]. Chinese Journal of Luminescence, 2013,34(11): 1538-1543 DOI： 10.3788/fgxb20133411.1538.

摘要

开发溶酶体荧光探针对于研究与溶酶体相关的细胞生理、病理活动具有重要的理论意义及实际应用价值。7-二乙胺基-3-(2'-吡啶)香豆素 (L3) 是一种弱碱性化合物

易于累积在酸性溶酶体中

有作为溶酶体荧光探针的潜质。本文详细研究了pH值对L3光谱性质的影响及其溶酶体定位成像能力。研究结果表明

该化合物具有一定的溶酶体定位能力

可以作为溶酶体荧光探针先导化合物。

Abstract

The study of lysosomal fluorescence probe has important theoretical significance and practical value for cell physiological and pathological research associated with lysosome. As a weak basic compound which prefer to the acid lysosome

7-(diethylamino)-3-(pyridin-2-yl)coumarin (L3) may be a potential lysosome -targetable fluorescent probe. The relationship between pH value and the spectroscopic properties of L3 were investigated as well as lysosome-targetable fluorescent imaging in this study. The results show that L3 can be used as a lead compounds for lysosome-targetable fluorescent probe.

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references

Li C, Greenwood T R, Glunde K. Glucosamine-bound near-infrared fluorescent probes with lysosomal specificity for breast tumor imaging [J]. Neoplasia, 2008, 10(4):389-398.[2] Shi H, He X X, Yuan Y, et al. Nanoparticle-based biocompatible and long-life marker for lysosome labeling and tracking [J]. Anal. Chem., 2010, 82(6):2213-2220.[3] Wu Z G, Tang M L, Tian T, et al. A specific probe for two-photon fluorescence lysosomal imaging [J]. Talanta, 2011, 87:216-221.[4] Fan F K, Nie S, Yang D M, et al. Labeling lysosomes and tracking lysosome-dependent apoptosis with a cell-permeable activity-based probe [J]. Bioconjugate Chem., 2012, 23(6):1309-1317.[5] Wu S Q, Li Z H, Han J H, et al. Dual colored mesoporous silica nanoparticles with pH activable rhodamine-lactam for ratiometric sensing of lysosomal acidity [J]. Chem. Commun., 2011, 47(40):11276-11278.[6] Vasylevska A S, Karasyov A A, Borisov S M, et al. Novel coumarin-based fluorescent pH indicators, probes and membranes covering a broad pH range [J]. Anal. Bioanal. Chem., 2007, 387(6):2131-2141.[7] Saleh N, Al-Soud Y A, Nau W M. Novel fluorescent pH sensor based on coumarin with piperazine and imidazole substi-tuents [J]. Spectrochim. Acta A, 2008, 71(3):818-822.[8] Yu T Z, Yang S D, Zhao Y L, et al. Synthesis, crystal structures and fluorescence properties of 3-(2-pyridyl)coumarin derivatives [J]. J. Photochem. Photobiol. A, 2010, 214(1):92-99.[9] Covington A K, Paabo M, Robinson R A, et al. Use of the glass electrode in deuterium oxide and the relation between the standardized pD(paD) scale and the operational pH in heavy water [J]. Anal. Chem., 1968, 40(4):700-706.[10] Sun H. Construct of pH fluorescent probes [D]. Nanjing: Nanjing University, 2011.[11] Liu C, Sun H, He W J, et al. A new benzothiazole-derived pH fluorescent sensor of large Stokes shift [J]. Chin. J. Inorg. Chem.(无机化学学报), 2011, 27(11):2121-2127 (in Chinese).[12] Baracca A, Sgarbi G, Solaini G, et al. Rhodamine 123 as a probe of mitochondrial membrane potential: Evaluation of proton flux through F0 during ATP synthesis [J]. Biochim. Biophys. Acta-Bioenerg., 2003, 1606(1-3):137-146.[13] Dickinson B C, Srikun D, Chang C J. Mitochondrial-targeted fluorescent probes for reactive oxygen species [J]. Curr. Opin. Chem. Biol., 2010, 14(1):50-56.

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