Study on Liver Cancer Cells (HepG2) Under Simulated Microgravity Based on Raman Spectroscopy

WANG Yan-jun; WANG Gui-wen; YAO Hui-lu

doi:10.3788/fgxb20133410.1408

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Study on Liver Cancer Cells (HepG2) Under Simulated Microgravity Based on Raman Spectroscopy

更新时间：2020-08-12

- Study on Liver Cancer Cells (HepG2) Under Simulated Microgravity Based on Raman Spectroscopy
- Chinese Journal of Luminescence Vol. 34, Issue 10, Pages: 1408-1411(2013)
- 作者机构：
  
  1. 中国科学院动物研究所膜工程与生物膜国家重点实验室细胞生物学系, 北京 100101
  2. 广西科学院生物物理实验室, 广西南宁 530003
- 作者简介：
- 基金信息：
- DOI：10.3788/fgxb20133410.1408
  CLC： Q631
- Received：01 July 2013，
  
  Revised：22 August 2013，
  
  Published：10 October 2013
- 稿件说明：
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王雁军, 王桂文, 姚辉璐. 基于拉曼光谱的微重力三维培养条件下的人HepG2细胞的研究[J]. 发光学报, 2013,34(10): 1408-1411

WANG Yan-jun, WANG Gui-wen, YAO Hui-lu. Study on Liver Cancer Cells (HepG2) Under Simulated Microgravity Based on Raman Spectroscopy[J]. Chinese Journal of Luminescence, 2013,34(10): 1408-1411
王雁军, 王桂文, 姚辉璐. 基于拉曼光谱的微重力三维培养条件下的人HepG2细胞的研究[J]. 发光学报, 2013,34(10): 1408-1411 DOI： 10.3788/fgxb20133410.1408.

WANG Yan-jun, WANG Gui-wen, YAO Hui-lu. Study on Liver Cancer Cells (HepG2) Under Simulated Microgravity Based on Raman Spectroscopy[J]. Chinese Journal of Luminescence, 2013,34(10): 1408-1411 DOI： 10.3788/fgxb20133410.1408.

摘要

采用一个波长为785 nm的半导体激光束来囚禁人的肝癌细胞并激发人的肝癌细胞的拉曼光谱

分析模拟微重力三维培养条件下的人肝癌细胞的DNA、蛋白和脂类的生物学物质的变化和表达情况。结果显示

微重力三维培养条件下和平面培养下的人的肝癌细胞的拉曼光谱在527

1 367

1 438

1 659 cm

-1

处有明显的差异。

Abstract

In our work

HepG2 was cultured onto biodegradable polyglycolic acid(PGA) polymer scaffolds

which was cultured in a rotating cell culture system (RCCS) to form a three-dimensional (3D) and human liver cancer cells cultured in common plate. We detected the spectra of HepG2 cells under two culture conditions and compared the spectra changes of the different cells under two culture conditions. The results show that Raman spectroscopy can differentiate among HepG2 cells under two culture conditions. 527 cm

-1

and 1 357 cm

-1

peaks of HepG2 cells under simulated microgravity are higher than that of HepG2 cells in common plate. 1 438 cm

-1

and 1 659 cm

-1

peaks of HepG2 cells under simulated microgravity are lower than that of HepG2 cells in common plate. It is known that 527 cm

-1

represents Fe(Ⅱ)

-MeIm

1 357 cm

-1

represents Guanine

porphyrins (haemoglobin)

lipids

1 438 cm

-1

represents CH

deformation (lipids)

cholesterol

and 1 659 cm

-1

represents Amide I: proteins. These biomaterials which induce these peak changes are possibly related to 3D culture.

关键词

Keywords

references

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