A Determination Method of Glipizide Content with Bovine Serum Albumin as Fluorescent Probe

CAO Shi-na; LIU Bao-sheng; LI Zhi-yun; CHONG Bao-hong

doi:10.3788/fgxb20133407.0930

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A Determination Method of Glipizide Content with Bovine Serum Albumin as Fluorescent Probe

更新时间：2020-08-12

- A Determination Method of Glipizide Content with Bovine Serum Albumin as Fluorescent Probe
- Chinese Journal of Luminescence Vol. 34, Issue 7, Pages: 930-934(2013)
- 作者机构：
  
  河北大学化学与环境科学学院,河北保定,071002
- 作者简介：
- 基金信息：
- DOI：10.3788/fgxb20133407.0930
  CLC： O657.3
- Received：09 April 2013，
  
  Revised：01 June 2013，
  
  Published：10 July 2013
- 稿件说明：
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曹世娜, 刘保生, 李志云, 种宝红. 以牛血清白蛋白为荧光探针的格列吡嗪含量测定方法[J]. 发光学报, 2013,34(7): 930-934

CAO Shi-na, LIU Bao-sheng, LI Zhi-yun, CHONG Bao-hong. A Determination Method of Glipizide Content with Bovine Serum Albumin as Fluorescent Probe[J]. Chinese Journal of Luminescence, 2013,34(7): 930-934
曹世娜, 刘保生, 李志云, 种宝红. 以牛血清白蛋白为荧光探针的格列吡嗪含量测定方法[J]. 发光学报, 2013,34(7): 930-934 DOI： 10.3788/fgxb20133407.0930.

CAO Shi-na, LIU Bao-sheng, LI Zhi-yun, CHONG Bao-hong. A Determination Method of Glipizide Content with Bovine Serum Albumin as Fluorescent Probe[J]. Chinese Journal of Luminescence, 2013,34(7): 930-934 DOI： 10.3788/fgxb20133407.0930.

摘要

在pH=7.40 的Tris-HCl缓冲溶液中

牛血清白蛋白(BSA)与格列吡嗪(Glipizide)的相互作用会使BSA荧光有规律地发生猝灭

据此建立了以BSA为探针的Glipizide含量测定方法。Glipizide浓度在0.25~20 mol/L范围内与荧光强度呈现良好的线性关系

方法的检出限为0.071 mol/L。对于片剂中Glipizide含量的测定

平行6次测定的相对标准偏差为1.29%~2.80%

回收率为86.79%~96.43%。

Abstract

In Tris-HCl buffer solution(pH=7.40)

the reaction between bovine serum albumin (BSA) and Glipizide can lead the fluorescence of BSA quenching regularly. Based on this pheno-menon

a new method that determining the content of Glipizide was established

in which BSA was used as the fluorescent probe. The concentration of Glipizide presented a nice liner relation with the fluorescence intensity from 0.25 to 20 mol/L. The detection limit was 0.071 mol/L. The method was applied to analysis the Glipizide content in drugs samples

and the RSD was from 1.29% to 2.80%

the recovery rate was from 86.79% to 96.43% with six parallel determinations.

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Keywords

references

Li X, Xu Z, Lu X, et al. Comprehensive two-dimensional gas chromatography/time-of-flight mass spectrometry for metabonomics: Biomarker discovery for diabetes mellitus[J]. Anal. Chim. Acta, 2009, 633(2):257-262.[2] AbuRuz S, Millership J, McElnay J. The development and validation of liquid chromatography method for the simultaneous determination of metformin and glipizide, gliclazide, glibenclamide or glimperide in plasma[J]. J. Chromatogr. B, 2005, 817(2):277-286.[3] Kradjan W A, Kobayashi K A, Bauer L A, et al. Glipizide pharmaco-kinetics:effects of age, diabetes, and multiple dosing[J]. J. Clin. Pharmaco., 1989, 29(12):1121-1127.[4] Li X M, Cao W, Wu J P, et al. Pharmacokinetics of compound glipizide and metformin hydrochloride tablets in healthy volunteers[J]. Chin. Hosp. Pharm. J.(中国医院药学杂志), 2007, 27(7):869-872 (in Chinese).[5] Li Q Y, Li X Z, Xu S P. Determination of the plasma concentration of glipizide by HPLC[J]. J. Clin. Res.(医学临床研究), 2005, 22(1):137-138 (in Chinese).[6] Yan S H. An HPLC method for the determination of the concentration of glipizide and the improvement of mobile phase on content uniformity[J]. Guide of China Medicine (中国医药指南), 2010, 8(28):50-51 (in Chinese).[7] Tang J L, Wang L, Xiang R F, et al. Determination of glipizide in human serum by HPLC[J]. Chin. J. Anal. Lab.(分析实验室), 2007, 26(2):84-88 (in Chinese).[8] Feng J, Liu J, Su D, et al. Determination of two components in metformin and glipizide tablets by reversed-phase ion-pair HPLC[J]. Chin. J. New Drugs Clin. Rem.(中国新药与临床杂志), 2010, 29(6):445-447 (in Chinese).[9] Tong R F, Qin X M, Ding L, et al. Analysis of urine excretion of metformin-glipizide tablet after oral administration in healthy human[J]. Tianjin Med. J.(天津医药), 2012, 40(1):7-11 (in Chinese).[10] Zhao X H, Song B, Zhong D F, et al. Simultaneous determination of metformin and glipizide in human plasma by liquid chromatography-tandem mass spectrometry[J]. Acta Pharmaceutica Sinica (药学学报), 2007, 42(10):1087-1091 (in Chinese).[11] Liu Y, Huang L J, Wang J D, et al. Simultaneous determination of three diabetic drugs in urine by high performance liquid chromatography[J]. J. Instrumental. Anal.(分析测试学报), 2011, 30(3):293-297 (in Chinese).[12] Liu B S, Yan X N, Cao S N, et al. Interaction of cefpiramide with bovine serum albumin and the effect of coexistent metal ion on the reaction[J]. Chin. J. Lumin.(发光学报), 2012, 33(9):1018-1024 (in Chinese).[13] Jiang T M, Tan F P, Du J L, et al. Preparation and in vitro release of glipizide loaded controlled release microspheres[J]. J. Tsinghua Univ.(Sci & Tech)(清华大学学报(自然科学版)), 2004, 44(6):732-735 (in Chinese).[14] Tian L F, Liu Z F, Hu X L, et al. Proteins fluorescence quenching by 2- and its analytical application[J]. Chem. J. Chin. Univ.(高等学校化学学报), 2012, 33(1):59-65 (in Chinese).[15] Zhang S, Nie S F, Wei L L, et al. Study on inclusive reaction of water-soluble -cyclodextrin polymer with glipizide[J]. Chin. Pham. J.(中国药学杂志), 2007, 42(8):601-604 (in Chinese).[16] Huang G W, Zhang C P. Suggestion on assay of glipizide tablets[J]. Drug Standard of China (中国药品标准), 2005, 6(2):52-53 (in Chinese).

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