Key Technologies of Chemiluminescence Immunoassay Analyzer

ZHAO Jian-wen; CHEN Yan-ning; LU Shi-zhou

doi:10.3788/fgxb20123312.1381

您当前的位置：

首页 >

文章列表页 >

Key Technologies of Chemiluminescence Immunoassay Analyzer

更新时间：2020-08-12

- Key Technologies of Chemiluminescence Immunoassay Analyzer
- Chinese Journal of Luminescence Vol. 33, Issue 12, Pages: 1381-1388(2012)
- 作者机构：
  
  1. 哈尔滨工业大学(威海)数字化医疗装备山东省重点实验室,山东威海,264209
  2. 哈尔滨医科大学附属三院检验科,黑龙江哈尔滨,150040
- 作者简介：
- 基金信息：
- DOI：10.3788/fgxb20123312.1381
  CLC： TH79
- Received：12 October 2012，
  
  Revised：26 October 2012，
  
  Published：10 December 2012
- 稿件说明：
移动端阅览
赵建文, 陈艳宁, 路士州. 化学发光免疫检测仪涉及的关键技术[J]. 发光学报, 2012,33(12): 1381-1388

ZHAO Jian-wen, CHEN Yan-ning, LU Shi-zhou. Key Technologies of Chemiluminescence Immunoassay Analyzer[J]. Chinese Journal of Luminescence, 2012,33(12): 1381-1388
赵建文, 陈艳宁, 路士州. 化学发光免疫检测仪涉及的关键技术[J]. 发光学报, 2012,33(12): 1381-1388 DOI： 10.3788/fgxb20123312.1381.

ZHAO Jian-wen, CHEN Yan-ning, LU Shi-zhou. Key Technologies of Chemiluminescence Immunoassay Analyzer[J]. Chinese Journal of Luminescence, 2012,33(12): 1381-1388 DOI： 10.3788/fgxb20123312.1381.

摘要

为实现化学发光免疫检测方法的临床应用

在深入分析化学发光免疫检测过程的基础上

对其涉及的各项关键技术进行了研究。针对化学发光免疫检测过程的3个关键环节:加样、洗涤和测量

提炼出化学发光免疫检测仪涉及的3项关键技术:微量液体试样精确加样技术、免疫复合物充分无损分离技术、微弱闪光信号精确测量技术。在液体加样方面

通过微升级别液体加注过程的分析及仿真给出加样模块相关参数的优化原则;在免疫复合物分离方面

通过复合物颗粒磁分离过程的分析给出磁场设计原则;在光检方面

给出了测量室设计原则及光电信号数据处理原则。在攻克上述关键技术的基础上

研制出Autolumis 3000化学发光免疫分析仪并成功实现临床应用。测试结果表明

仪器的变异系数 (Coefficient of variance

CV)为5%

最大检测速度为每小时180次

达到国外同类高端仪器的水平

表明所研究的关键技术是可行的。

Abstract

To apply the chemiluminescence immunoassay method in clinical applications

some key technologies were investigated based on deeply analyzing the chemiluminescence immunoassay process. Firstly

three key steps about the immunoassay were indicated: filling-up

washing and measuring. Accordingly

three key technologies were summarized: accurate filling-up of microcontent liquid

quick and effective separation of immune complex

and accurate measurement of weak optical signal. In the aspect of filling-up

based on the analysis and simulation of milliliter level liquid filling-up process

the principle for optimizing relative sampling parameter was obtained. In the aspect of washing

the principle for designing the magnetic field was then obtained based on the analysis of separating immune complex process. In the aspect of measuring

the principles for designing measuring room and processing optical signal were given. After investigating the above-mentioned key technologies

chemiluminescence immunoassay analyzer Autolumis 3000 has been developed and applied in hospital. The results show that the coefficient of variance (CV) is 5% and the maximum speed is 180 time/h. This analyzer can achieve similar performance comparing with the current high-end equipment in abroad

which validates our investigation of the key technologies.

关键词

Keywords

references

Kim S Y, Kim J H, Yoon S Y, et al. Clinical performance evaluation of four automated chemiluminescence immunoassays for hepatititis C virus antibody detection [J]. J. Clinical Microbiology, 2008, 46(12):3919-3923.

Luo G Y, Wang B D, Yuan C H, et al. The key technologies of a new semi-automatic chemiluminescence immunoassay analyzer based on the photomultiplier tube // 2010 3rd International Conference on Advanced Computer Theory and Engineering, Chendu: IEEE, 2010, 6:402-405.

Wang Y, Lin J M. Chemiluminescence immunoassay technology and new advances [J]. Chin. J. Anal. Lab. (光谱实验室), 2007, 26(6):111-122 (in English).

Michels D A, McElroy W, Voehringer D. Charge heterogeneity of monoclonal antibodies by multiplexed imaged capillary isoelectric focusing immunoassay with chemiluminescence detection [J]. Anal. Chem., 2012, 84(12):5380-5386.

Vdovenko M M, Gribas A V, Vylegzhanina A V. Development of a chemiluminescent enzyme immunoassay for the determination of dexamethasone in milk [J]. Anal. Methods, 2012, 4(8):2550-2554.

Natrajan A, Sharpe D, Wen D. Zwitterionic reagents for labeling, cross-linking and improving the performance of chemiluminescent immunoassays [J]. Org. Biomol. Chem., 2012, 10(9):1883-1895.

Hara T, Toriyama M, Tsukagoshi K. Immunoassay using a metal-complex compound as a chemiluminescent catalyst [J]. Bull. Chem. Soc. Jpn., 1983, 56(10):2965-2968.

Klinger W, Strasburger C J, Wood W G. Chemiluminescent tags in immunoassays [J]. TrAC Trends Anal. Chem., 1983, 2(6):132-136.

Liu Y X, Chen L G, Sun L N. Non-contact liquid rationing system based on compound intelligent control [J]. J. Mech. Eng.(机械工程学报), 2010, 46(20):175-181 (in Chinese).

Yang M, Zhu L, Hou L Y, et al. Design and experiment of vitreous combined micronozzles used in digital micro injection [J]. Opt. Precision Eng.(光学精密工程), 2012, 20(7):1580-1586 (in Chinese).

Ueda H, Agatsuma K, Fuchino S, et al. Improvement of a high-gradient magnetic separation system for trapping immunoglobulin in serum [J]. Appl. Supercond., 2010, 20(3):949-952.

Views

327

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Research Progresses on Chemiluminescent Gold Nanoclusters Applied in Biological in vitro Detection

Research Advances in Carbon Dot-based Luminol and Peroxyoxalate Chemiluminescent Systems

Effect of Polyphenol Compounds on Luminol-AgNO₃-gold Nanoparticles Chemical Luminescence System

Hypersensitization Immunoassay of Prostate-specific Antigen Based on SERS of Sandwich-type Au/Ag Nanostructure

Determination of Hydroquinone in The Aquatic Environment by Chemiluminescence of Luminol-H₂O₂ Sensitized by CdTe Quantum Dots

Related Author

Xian-ping FAN

Xu-sheng QIAO

Dong CHEN

Shi-sheng LING

Xiao-ru DAI

Yun-peng WANG

Tian-hao XIA

Chong-xin SHAN

Related Institution

Assure Tech. (Hangzhou) Co., LTD.

State Key Laboratory of Silicon Materials & School of Materials Science and Engineering, Zhejiang University

Key Laboratory of Materials Physics, Ministry of Education, School of Physics and Microelectronics, Zhengzhou University

Shaanxi Normal University

Institute of Technology, Henan Xinyang University

AI问答

Address：No.3888 Dong Nanhu Road, Changchun, Jilin, China Postal code：130033
Tel：0431-86176862 Email：fgxbt@126.com
Technical support is provided by Beijing Founder electronics co., LTD 吉ICP备11002662号-17 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰