Study on Interaction of Y3+ and Bovine Serum Albumin by Spectrometry

WU Jin-xiu; LI Mei; LIU Zhao-gang; HU Yan-hong; WANG Mi-tang

doi:10.3788/fgxb20123310.1153

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Study on Interaction of Y³⁺ and Bovine Serum Albumin by Spectrometry

更新时间：2020-08-12

- Study on Interaction of Y³⁺ and Bovine Serum Albumin by Spectrometry
- Chinese Journal of Luminescence Vol. 33, Issue 10, Pages: 1153-1159(2012)
- 作者机构：
  
  1. 内蒙古科技大学稀土学院,内蒙古包头,014010
  2. 内蒙古自治区高校稀土现代冶金新技术与应用重点实验室,内蒙古包头,014010
- 作者简介：
- 基金信息：
- DOI：10.3788/fgxb20123310.1153
  CLC： O657.3;O482.31
- Received：24 May 2012，
  
  Revised：25 July 2012，
  
  Published：10 October 2012
- 稿件说明：
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吴锦绣, 李梅, 柳召刚, 胡艳宏, 王觅堂. 光谱法研究稀土离子钇(Ⅲ)与牛血清白蛋白的相互作用[J]. 发光学报, 2012,(10): 1153-1159

WU Jin-xiu, LI Mei, LIU Zhao-gang, HU Yan-hong, WANG Mi-tang. Study on Interaction of Y3+ and Bovine Serum Albumin by Spectrometry[J]. Chinese Journal of Luminescence, 2012,(10): 1153-1159
吴锦绣, 李梅, 柳召刚, 胡艳宏, 王觅堂. 光谱法研究稀土离子钇(Ⅲ)与牛血清白蛋白的相互作用[J]. 发光学报, 2012,(10): 1153-1159 DOI： 10.3788/fgxb20123310.1153.

WU Jin-xiu, LI Mei, LIU Zhao-gang, HU Yan-hong, WANG Mi-tang. Study on Interaction of Y3+ and Bovine Serum Albumin by Spectrometry[J]. Chinese Journal of Luminescence, 2012,(10): 1153-1159 DOI： 10.3788/fgxb20123310.1153.

摘要

用荧光光谱和紫外-可见吸收光谱研究了稀土金属离子Y

与牛血清白蛋白(BSA)的相互作用。实验结果发现:Y

对BSA的紫外吸收光谱具有增强作用

而对荧光光谱具有较强的荧光猝灭作用且峰位明显蓝移20~25nm。用Stern-Volmer方程分别对实验数据进行分析

得出结论:Y

对BSA的荧光猝灭作用是属于静态荧光猝灭

与BSA反应生成了新的复合物

发生了分子内的非辐射能量转移。求得相互作用过程的结合常数(

)和热力学参数(Δ

、Δ

)

确定了它们之间的主要作用力是范德华力、氢键等

但静电作用力也不可忽略。同步荧光光谱法表明Y

对牛血清白蛋白的构象有影响。

Abstract

The interaction between rare earth ion Y

and bovine serum albumin(BSA) was studied by fluorescence spectrum and ultraviolet-visible absorption spectrometry.It was shown that Y

has a quite strong effect to quench the fluorescence launching and enhance the UV absorption spectra of BSA.The maximum emission peak of BSA shifted to short wave by 20~25 nm.After the fluorescence quenching date was analyzed by Stern-Volmer equation

the results indicated that the reaction between bovine serum albumin and Y

generated the new complex-system.The quenching belonged to static fluorescence quenching

with non-radiation energy transfer happening within single molecule.The binding constants (

) and thermodynamics parameters (Δ

) were calculated according to equation of fluorescence spectrometry at different temperatures.Based on thermodynamic data

the main reaction between Y

and BSA was van der Waals force and hydrogen bond

but electrostatic force can not be ignored.The effect of rare earth ion Y

on the conformation of BSA was researched by synchronous fluorescence spectrometry.

关键词

Keywords

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Ross D P,Subramanian S.Themodynamics of protei association reactions forces contributing to stability [J]. Biochemistry,1981,20(11):3096-3103.《发光学报》网上在线投稿通知由于学报发展的需要,《发光学报》网站已经建成开通,欢迎广大作者浏览我们的网页并提出宝贵意见,共同建好这个为广大作者和读者进行交流以及展示作者相关科研成果的平台。《发光学报》网页上建有网上在线投稿平台,我们只接收网上在线投稿,欢迎大家使用。如有问题,请与我们联系:E-mail:fgxbt@126.com,Tel:(0431)86176862,84613407《发光学报》网址:http://www.fgxb.org 《发光学报》编辑部《发光学报》第10届编辑委员会委员名单 The 10th Editorial Committee of Chinese Journal of Luminescence (2010.11~2013.10) 名誉主编徐叙瑢院士北京交通大学 E-mail:xrxu@bjtu.edu.cn范希武研究员中国科学院长春光学精密机械与物理研究所 E-mail:xiwufan@163.com 顾问 Prof.MELTZER Richard S Department of Physics and Astronomy,The University of Georgia,USAProf.YOKOYAMA Meiso Department of Electronic Engineering,I-Shou University,Taiwan,China刘洪楷研究员中国科学院长春光学精密机械与物理研究所主编申德振研究员中国科学院长春光学精密机械与物理研究所 E-mail:shendz@ciomp.ac.cn 副主编侯晓远教授复旦大学 E-mail:xyhou@fudan.edu.cn刘益春教授东北师范大学 E-mail:ycliu@nenu.edu.cn许武教授中国科学技术大学 E-mail:xuw@ustc.edu.cn张洪杰研究员中国科学院长春应用化学研究所 E-mail:hongjie@ciac.jl.cn付国柱 (常务) 中国科学院长春光学精密机械与物理研究所 E-mail:fgxbt@126.com 编委陈登铭教授台湾交通大学 E-mail:tmchen@mail.nctu.edu.tw侯延冰教授北京交通大学 E-mail:ybhou@bjtu.edu.cn蒋大鹏研究员中国科学院长春光学精密机械与物理研究所 E-mail:jdp@ciomp.ac.cn江风益教授南昌大学 E-mail:jiangfengyi@latticepower.com李斌研究员中国科学院长春光学精密机械与物理研究所 E-mail:lib020@ciomp.ac.cn梁宏斌教授中山大学 E-mail:cesbin@mail.sysu.edu.cn林君研究员中国科学院长春应用化学研究所 E-mail:jlin@ciac.jl.cn刘宝林教授厦门大学 E-mail:blliu@xmu.edu.cn吕有明教授深圳大学 E-mail:ymlu@szu.edu.cn彭俊彪教授华南理工大学 E-mail:psjbpeng@scut.edu.cn邱勇教授清华大学 E-mail:qiuy@mail.tsinghua.edu.cn申泽骧教授新加坡国立大学 E-mail:zexiang@ntu.edu.sg宋宏伟教授吉林大学 E-mail:hwsong2005@yahoo.com汤子康教授香港科学技术大学 E-mail:phzktang@ust.hk王启明院士中国科学院半导体研究所 E-mail:qmwamg@red.semi.ac.cn王晓华教授长春理工大学 E-mail:biewang@126.com王笑军教授 Dept.Physics,Georgia Southern University E-mail:xwamg.gsu@gmail.com王永生教授北京交通大学 E-mail:yshwang@bjtu.edu.cn王育华教授兰州大学 E-mail:wyh@lzu.edu.cn夏建白院士中国科学院半导体研究所 E-mail:xiajb@red.semi.ac.cn严纯华院士北京大学 E-mail:yan@pku.edu.cn杨志平教授河北大学 E-mail:yangzhiping786@sohu.com尹民教授中国科学技术大学 E-mail:yinmin@ustc.edu.cn印寿根教授天津理工大学 E-mail:sgyin@tjut.edu.cn张国义教授北京大学 E-mail:gyzhang@pku.edu.cn郑海荣教授陕西师范大学 E-mail:hrzheng@snnu.edu.cn

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