Synthesis and Interaction of Succinic-oligochitosan-Eu(Ⅲ) Complex with Bovine Serum Albumin

LI Xiao-fang; FENG Xiao-qiang; YANG Sheng; ZHANG Yu-xia

doi:10.3788/fgxb20123308.0905

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Synthesis and Interaction of Succinic-oligochitosan-Eu(Ⅲ) Complex with Bovine Serum Albumin

更新时间：2020-08-12

- Synthesis and Interaction of Succinic-oligochitosan-Eu(Ⅲ) Complex with Bovine Serum Albumin
- Chinese Journal of Luminescence Vol. 33, Issue 8, Pages: 905-910(2012)
- 作者机构：
  
  1. 天水师范学院生命科学与化学学院,甘肃天水,741001
  2. 定西师范高等专科学校,甘肃定西,743000
- 作者简介：
- 基金信息：
- DOI：10.3788/fgxb20123308.0905
  CLC： O614.338
- Received：07 May 2012，
  
  Revised：03 June 2012，
  
  Published：10 August 2012
- 稿件说明：
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李小芳, 冯小强, 杨声, 张玉霞. 丁二酰化壳寡糖铕配合物的合成及与牛血清白蛋白的相互作用[J]. 发光学报, 2012,33(8): 905-910

LI Xiao-fang, FENG Xiao-qiang, YANG Sheng, ZHANG Yu-xia. Synthesis and Interaction of Succinic-oligochitosan-Eu(Ⅲ) Complex with Bovine Serum Albumin[J]. Chinese Journal of Luminescence, 2012,33(8): 905-910
李小芳, 冯小强, 杨声, 张玉霞. 丁二酰化壳寡糖铕配合物的合成及与牛血清白蛋白的相互作用[J]. 发光学报, 2012,33(8): 905-910 DOI： 10.3788/fgxb20123308.0905.

LI Xiao-fang, FENG Xiao-qiang, YANG Sheng, ZHANG Yu-xia. Synthesis and Interaction of Succinic-oligochitosan-Eu(Ⅲ) Complex with Bovine Serum Albumin[J]. Chinese Journal of Luminescence, 2012,33(8): 905-910 DOI： 10.3788/fgxb20123308.0905.

摘要

合成并表征了丁二酰化壳寡糖铕配合物

在模拟人体生理条件下

运用紫外和荧光光谱研究了配合物与牛血清白蛋白(BSA)的相互作用。结果表明:随着配合物浓度的增加

BSA的紫外光谱表现出增色效应并伴有蓝移;配合物与BSA形成复合物

从而猝灭BSA内源荧光

该猝灭机制为静态猝灭。计算得到室温下配合物与BSA的结合常数和结合位点数分别为2.068 2×10

L·mol

-1

和1.094 72。

Abstract

Succinic-oligochitosan-Eu(Ⅲ) complex was synthesized and characterized. Under simulated physiological conditions

the interaction between complex and bovine serum albumins (BSA) was studied by ultraviolet spectrum and fluorescence spectrum. The results showed that the absorption intensity of BSA increased with the concentration of complex. The complex quenched the fluorescence of BSA and it was static quenching

and then the binding constant and binding sites were calculated . They were 2.068 2×10

L·mol

-1

and 1.094 72 at room temperature

respectively.

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Keywords

references

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