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大连海事大学 信息科学技术学院,辽宁 大连,116026
Received:15 February 2011,
Revised:20 April 2011,
Published Online:22 July 2011,
Published:22 July 2011
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李瑛, 许文海. 利用延迟荧光光谱<em>F</em><sub>730</sub>/<em>F</em><sub>685</sub>的逆境胁迫生理检测方法[J]. 发光学报, 2011,32(7): 740-747
LI Ying, XU Wen-hai. Detection of Stress Physiology of Plant Using <em>F</em><sub>730</sub>/<em>F</em><sub>685</sub> Ratio of Delayed Fluorescence Spectrum[J]. Chinese Journal of Luminescence, 2011,32(7): 740-747
李瑛, 许文海. 利用延迟荧光光谱<em>F</em><sub>730</sub>/<em>F</em><sub>685</sub>的逆境胁迫生理检测方法[J]. 发光学报, 2011,32(7): 740-747 DOI: 10.3788/fgxb20113207.0740.
LI Ying, XU Wen-hai. Detection of Stress Physiology of Plant Using <em>F</em><sub>730</sub>/<em>F</em><sub>685</sub> Ratio of Delayed Fluorescence Spectrum[J]. Chinese Journal of Luminescence, 2011,32(7): 740-747 DOI: 10.3788/fgxb20113207.0740.
以玉米98-2为样品
实验分析了高温、盐及紫外UV-B胁迫下
样品离体叶片延迟荧光发射光谱的变化。标记685 nm主峰带的最高峰值为
F
685
730 nm次峰带的最高峰值为
F
730
实验发现不同的逆境胁迫对比值
F
730
/
F
685
均产生了显著的影响。结合样品在相同逆境下叶绿素荧光参数
F
v
/
F
m
以及DF强度的检测实验
结果表明
无论是在哪种逆境胁迫下
无论是对胁迫程度还是胁迫时间的响应特性上
F
730
/
F
685
、DF强度与
F
v
/
F
m
均有着较好的一致性
线性拟合相似度大于0.960 9。因此
通过对本文实验结果的分析认为
延迟荧光光谱峰值比
F
730
/
F
685
的逆境胁迫响应与叶片受胁迫影响的程度密切相关
且可能是一种检测植物逆境光合生理的新方法。
Delayed fluorescece (DF) is intrinsically related to the photosynthesis process
and it is a good method of researching the effect of various chemical and physical factors in vivo and in vitro on photosynthetic performance of plants. In this paper
the detection of DF emission spectra under the stress (heat
salt and UV-B) have been investigated in maize (98-2) sample. The maximal values of DF emission spectra at 685 nm and 730 nm were marked as
F
685
and
F
730
respectively. After comparative measurement of
F
730
/
F
685
ratio
DF intensity and
F
v
/
F
m
the linear fitting results shown that the two parameters has a good linear correlation (
R
2
0.960 9). The experimental results suggest that the degree of plant photosynthetic physiology damage whether caused by different concentration or different stress time can be effectively measured by
F
730
/
F
685
. Moreover
the responses of
F
730
/
F
685
ratio of DF emission spectra to the stress could be a new and useful judgment to identify and evaluate the stress damage of the samples.
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