SUN Gui-min, YANG Pei-hui, SUN Jun-huan, CAI Ji-ye. Fluorescence Imaging of Hepatocellular Carcinoma Cells Labeled with Gold Nanorods and Detection by AFM[J]. Chinese Journal of Luminescence, 2011,32(6): 636-641
SUN Gui-min, YANG Pei-hui, SUN Jun-huan, CAI Ji-ye. Fluorescence Imaging of Hepatocellular Carcinoma Cells Labeled with Gold Nanorods and Detection by AFM[J]. Chinese Journal of Luminescence, 2011,32(6): 636-641 DOI: 10.3788/fgxb20113206.0636.
Fluorescence Imaging of Hepatocellular Carcinoma Cells Labeled with Gold Nanorods and Detection by AFM
gold nanorods have potential and important applications in the biomedical field. In this paper
gold nanorods with the aspect ratio 8 were prepared
an outstanding fluorescence feature of the gold nanorods excited at 480 nm was observed in terms of a strong fluorescence emission at 707 nm and a relatively weak fluorescence at 560 nm. Based on their fluorescence properties
HepG2 cells were labeled by this type of gold nanorods
and were studied by using laser scanning confocal microscope. Green and red fluorescence images of gold nanorods were collected under the excitation of 488 nm. Furthermore
high-resolution atomic force microscopy was used to obtain the morphology and mechanical properties changes of the HepGz cells.
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Huang X H, El-sayed I H, Qian W, et al. Cancer cells assemble and align gold nanorods conjugated to antibodies to produce highly enhanced, sharp, and polarized surface Raman spectra: A potential cancer diagnostic marker [J]. Nano. Lett., 2007, 7 (6):1591-1597.[2] Li C Z, Male K B, Hrapovic S, et al. Fluorescence properties of gold nanorods and their application for DNA biosensing [J]. Chem. Commun., 2005 (31):3924-3926.[3] Eustis S, El-Sayed M. Aspect ratio dependence of the enhanced fluorescence intensity of gold nanorods: experimental and simulation study [J]. J. Phys. Chem. B, 2005, 109 (34):16350-16356.[4] Fu Yanpeng, Li Ming, Zhou Qiang, et al. Facile synthesis and spectroscopic properties of chitosan /LaF3 ∶ Eu3+ nanocomposite particles [J]. Chin. J. Lumin. (发光学报), 2009, 30 (1):91-96 (in Chinese).[5] Li Yuejuan, Meng Jianxin, Cao Liwei, et al. Preparation and properties of near-infrared Yb(P4)(CoP) dyes doped polystyrene/silica nanopartickes [J]. Chin. J. Lumin. (发光学报), 2009, 30 (3):351-355 (in Chinese).[6] Fu Meiyan, Xiao Yi. The synthesis and application of a new thiocyanate sensor based on naphthalimide [J]. Chin. J. Lumin. (发光学报), 2009, 30 (4):482-486 (in Chinese).[7] Zhang Youlin, Zeng Qinghui, Kong Xianggui. The influence of bioconjugate process on the photoluminescence properties of water-soluble CdSe/ZnS core-shell quantum dots capped with polymer [J]. Chin. J. Lumin. (发光学报), 2010, 31 (1):101-104 (in Chinese).[8] Wang H F, Huff T B, Zweifel D A, et al. In vitro and in vivo two-photo luminescence imaging of single gold nanorods [J]. Proc. Natl. Acad. Sci. USA, 2005, 102 (44):15752-15756.[9] Durr N J, Larson T, Smith D K, et al. Two-photo luminescence imaging of cancer cells using molecularly targeted gold nanorods [J]. Nano Lett., 2007, 7 (4):941-945.[10] Huang X H, El-Sayed I H, Qian W, et al. Cancer cell imaging and photothermal therapy in the near-infrared region by using gold nanorods [J]. J. Am. Chem. Soc., 2006, 128 (6):2115-2120.[11] Oyelere, A K, Chen P C, Huang X, et al. Peptide-conjugated gold nanorods for nuclear targeting [J]. Bioconjug. Chem., 2007, 18 (5):1490-1497.[12] You H X, Lau J M, Zhang S, et al. Atomic force microscopy imaging of living cells: a preliminary study of the disruptive effect of the cantilever tip on cell morphology [J]. Ultramicroscopy, 2000, 82 (1-4):297-305.[13] Dvorak J A. The application of atomic force microscopy to the study of living vertebrate cells in culture [J]. Methods. 2003, 29 (1):86-96.[14] Barattin R, Voyer N. Chemical modifications of AFM tips for the study of molecular recognition events [J]. Chem. Commun., 2008 (13):1513-1532.[15] Nikoobakht B, El-Sayed M A. Preparation and growth mechanism of gold nanorods (NRs) using seed-mediated growth method [J]. Chem. Mater., 2003, 15 (10):1957-1962.[16] Boyd G T, Yu Z H, Shen Y R. Photoinduced luminescence from the noble metals and its enhancement on roughened surfaces [J]. Phys. Rev. B, 1986, 33 (12):7923-7936.