AODENG Gaowa, JIN Ying-chun. Spectroscopic Studies on The Interaction Between Fenbufen and Bovine Serum Albumin[J]. Chinese Journal of Luminescence, 2011,32(4): 404-410
AODENG Gaowa, JIN Ying-chun. Spectroscopic Studies on The Interaction Between Fenbufen and Bovine Serum Albumin[J]. Chinese Journal of Luminescence, 2011,32(4): 404-410 DOI: 10.3788/fgxb20113204.0404.
Spectroscopic Studies on The Interaction Between Fenbufen and Bovine Serum Albumin
The interaction between Fenbufen and bovine serum albumin(BSA) was studied with fluorescence spectra and UV-visible absorption spectra in the presence of simulating physiological systems.It showed that the complex formated by BSA and Fenbufen lead to the static quenching of the intrinsic fluorescence of BSA. The binding site number n and apparent binding constant
K
A
were measured according to Stern-Volmer equation.The distance between BSA and Fenbufen was obtained based on the Frster nonradiative energy transfer theory. We also confirmed that the main sorts of binding force between Fenbufen and BSA is electrostatic force. Meanwhile
synchronous fluorescence and three-dimensional fluorescence spectra were used to investigate the structure change of BSA before and after the introduction of Fenbufen.Furthermore
the effect of coexistence metal ions on the binding constants of Fenbufen with BSA was also discussed.
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