A Novel Curcumin Derivatives Fluorescent Probe for Selective Recognition of Cysteine

Fan LUO; Dong-bin ZHENG; Zhi-qiang YANG; Yun DENG

doi:10.37188/CJL.20210211

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A Novel Curcumin Derivatives Fluorescent Probe for Selective Recognition of Cysteine

Luminescence Applications and Interdisciplinary Fields | 更新时间：2021-11-23

- A Novel Curcumin Derivatives Fluorescent Probe for Selective Recognition of Cysteine
  增强出版
- Chinese Journal of Luminescence Vol. 42, Issue 11, Pages: 1810-1817(2021)
- 作者机构：
  
  成都中医药大学药学院　西南特色中药资源国家重点实验室，四川　成都　611137
- 作者简介：
- 基金信息：
  
  National Natural Science Foundation of China(U19A2011);Project First-Class Disciplines Development supported by Chengdu University of TCM(CZYJC1905)
- DOI：10.37188/CJL.20210211
  CLC： O482.31
- Received：18 June 2021，
  
  Revised：28 June 2021，
  
  Published：01 November 2021
- 稿件说明：
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Fan LUO, Dong-bin ZHENG, Zhi-qiang YANG, et al. A Novel Curcumin Derivatives Fluorescent Probe for Selective Recognition of Cysteine[J]. Chinese journal of luminescence, 2021, 42(11): 1810-1817.
DOI：

Fan LUO, Dong-bin ZHENG, Zhi-qiang YANG, et al. A Novel Curcumin Derivatives Fluorescent Probe for Selective Recognition of Cysteine[J]. Chinese journal of luminescence, 2021, 42(11): 1810-1817. DOI： 10.37188/CJL.20210211.

摘要

半胱氨酸(Cysteine

Cys)作为生物体内一种重要的含巯基氨基酸，与细胞代谢、氧化还原稳态密切相关，然而过量的半胱氨酸可能会导致类风湿性关节炎、阿尔兹海默病等。因此，开发一种能选择性识别半胱氨酸的荧光探针具有重要的研究意义。本文设计合成了一种以姜黄素-二氟硼为基本骨架，2-氯-5-硝基苯甲酰基为识别位点的荧光探针分子1，并将其用于半胱氨酸的识别。结果表明，探针能高选择性识别Cys，荧光强度与Cys浓度在10~70 μmol·L

-1

范围内呈良好的线性关系，检出限为2.9 μmol·L

-1

。同时，Cys的加入会引起探针溶液颜色肉眼可见的显著变化，由黄色变为无色。此外，该探针能模拟检测水样中添加的半胱氨酸，在自来水、河水样品中的半胱氨酸的回收率为98%~109%。

Abstract

As an important sulfhydryl amino acid in organism

cysteine(Cys) is closely related to cell metabolism and redox homeostasis. However

excess amount of Cys is associated with diseases including rheumatoid arthritis

Alzheimer's disease. Thus

it is of great significance to develop a fluorescent probe for selective recognition of cysteine. In this paper

a fluorescence probe based on curcumin-difluoride and modified with 2-chloro-5-nitrobenzoyl was synthesized for the determination of cysteine. The results showed that the probe could selectively recognize Cys. A good linear relationship between the fluorescence intensity of the probe and Cys concentration in the range of 10-70 μmol·L

-1

was obtained

from which the detection limit was deduced to be 2.9 μmol·L

-1

. Meanwhile

the addition of Cys could lead to an obvious colour transition from yellow to colourless

permitting a rapid identification of Cys by the naked eye. Moreover

the probe can simulate the detection of Cys in water samples

and the recoveries of Cys in tap water and river samples ranged from 98%-109%.

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Keywords

references

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