The Influence of Bioconjugate Process on the Photoluminescence Properties of Water-soluble CdSe/ZnS Core-shell Quantum Dots Capped with Polymer

ZHANG You-lin; ZENG Qing-hui; KONG Xiang-gui

doi:null

您当前的位置：

首页 >

文章列表页 >

The Influence of Bioconjugate Process on the Photoluminescence Properties of Water-soluble CdSe/ZnS Core-shell Quantum Dots Capped with Polymer

paper | 更新时间：2020-08-12

- The Influence of Bioconjugate Process on the Photoluminescence Properties of Water-soluble CdSe/ZnS Core-shell Quantum Dots Capped with Polymer
- Chinese Journal of Luminescence Vol. 31, Issue 1, Pages: 101-104(2010)
- 作者机构：
  
  1. 中国科学院研究生院北京,100049
  2. 中国科学院长春光学精密机械与物理研究所激发态物理重点实验室, 吉林长春 130033
- 作者简介：
- 基金信息：
- DOI：
  CLC： O482.31
- Received：08 May 2009，
  
  Revised：1900-1-2，
  
  Published Online：20 February 2010，
  
  Published：20 February 2010
- 稿件说明：
移动端阅览
ZHANG You-lin, ZENG Qing-hui, KONG Xiang-gui. The Influence of Bioconjugate Process on the Photoluminescence Properties of Water-soluble CdSe/ZnS Core-shell Quantum Dots Capped with Polymer[J]. Chinese Journal of Luminescence, 2010, 31(1): 101-104.
DOI：

ZHANG You-lin, ZENG Qing-hui, KONG Xiang-gui. The Influence of Bioconjugate Process on the Photoluminescence Properties of Water-soluble CdSe/ZnS Core-shell Quantum Dots Capped with Polymer[J]. Chinese Journal of Luminescence, 2010, 31(1): 101-104. DOI：

摘要

以传统的戊二醛交联的方法实现了聚丙烯酸包覆的CdSe/ZnS量子点和人IgG蛋白分子的偶联

同时研究了偶联过程对量子点发光性能的影响。通过琼脂糖凝胶电泳技术证明量子点和蛋白分子偶联成功。通过稳态光谱和时间分辨光谱研究了偶联过程对量子点荧光性质的影响。发现偶联到量子点表面的戊二醛分子能够破坏量子点的表面从而增加其表面缺陷

使量子点发光效率降低;上述产物进一步与人IgG偶联增强了量子点的荧光强度

这是由于连接到量子点上的蛋白分子修复了量子点的表面

从而降低了表面缺陷所致。

Abstract

Conjugates of human IgG and CdSe/ZnS quantum dots (QDs) capped with an amphiphilic poly (acrylic acid) polymer modified with poly (ehylene glycol) amine were synthesized by a glutaraldehyde (Cho) cross-linking procedure. The IgG-QD assemblies form in this reaction

as evidenced by gel electrophoresis. The key function of QD bioconjugates is to fluoresce readily and remain stable in biological conditions in order to allow for efficient bioapplication. However

the photoluminescence (PL) properties of QDs are sensitive to their environment

so it is particular important to study the mechanisms of the PL change of QDs linked to biomolecules. The effect of cross-linking processes on the PL of QDs was studied by means of steady-state and time-resolved photoluminescence spectroscopy. Attachment of Cho on the surface of QDs results in a significant decrease of QD emission due to the increase of surface defects with Cho destroying the surface of QDs. Attachment of IgG on the surface of QDs results in a significant increase of QD-Cho emission

which is attributed to the conjugation of IgG to QD modifying the surface of CdSe/ZnS QDs.

关键词

Keywords

references

. Chan W C, Nie S M. Quantum dot bioconjugates for ultrasensitive nonisotopic detection [J]. Science, 1998, 281 (5385):2016-2018.

. Bruchez M, Moronne M, Alivisatos A P, et al. Semiconductor nanocrystals as fluorescent biological labels [J]. Science, 1998, 281 (5385):2013-2016.

. Dubertret B, Skourides P, Norris D J, et al. In vivo imaging of quantum dots encapsulated in phospholipid micelles [J]. Science, 2002, 298 (5599):1759-1762.

. Jaiswal J K, Mattoussi H, Mauro J M, et al. Long-term multiple color imaging of live cells using quantum dot bioconjugates [J]. Nat. Biotechnol., 2003, 21 (1):47-51.

. Dahan M, Levi S, luccardini C, Rostaing P, et al. Diffusion dynamics of glycine receptors revealed by single-quantum dot tracking [J]. Science, 2003, 302 (5644):442-445.

. Kim S, Lim Y T, Soltesz E G, et al. Near-infrared fluorescent type quantum dots for sentinel lymph node mapping [J]. Nat. Biotechnol., 2004, 22 (1):93-97.

. Wu X, Liu H, Liu J, et al. Immunofluorescent labeling of cancer marker Her2 and other cellular targets with semicon-ductor quantum dots [J]. Nat. Biotechnol., 2003, 21 (1):41-46.

. Mamedova N N, Kotov N A, Rogach A L, et al. Albumin-CdTe nanoparticle bioconjugates: preparation, structure, and interunit energy transfer with antenna effect [J]. Nano Lett., 2001, 1 (6):281-286.

. Mattoussi H, Mauro J M, Goldman E R, et al. Self-assembly of CdSe-ZnS quantum dots bioconjugates using an engineered recombinant protein [J]. J. Am. Chem. Soc., 2000, 122 (49):12142-12150.

. Gerion D, Chen F, Kannan B, et al. Room-temperature single-nucleotide polymorphism and multiallele DNA detection using fluorescent nanocrystals and microarrays [J]. Anal. Chem., 2003, 75 (18):4766-4772.

. Daboussi B O, Viejo J R, Mikulec f V, et al. CdSe-ZnS core-shell quantum dots; synthesis and characterization of a size series of highly luminescnent nanocrystallites [J]. J. Phys. Chem. B, 1997, 101 (46):9463-9475.

. Xie R, Kolb U, Li J, Basche T, et al. Synthesis and characterization of highly luminescent CdSe-core CdS/ZnCdS/ZnS mutishell nanocrystals [J]. J. Am. Chem. Soc., 2005, 127 (20):7480-7488.

. Feng Liyun, Kong Xianggui. Preparation and characterization of fluorescence CdSe-liposome compound [J]. Chin. J. Lumin. (发光学报), 2007, 28 (3):417-420 (in Chinese).

. Jones M, Nedeljkovic J, Ellingson, et al. Photoenhancement of luminescence in colloidal CdSe quantum dot solution [J]. J. Phys. Chem. B, 2003, 107 (41):11346-11352.

. Hess B C, Okhrimenko I G, Davis R C, et al. Surface transformation and photoinduced recovery in CdSe nanocrystals [J]. Phys. Rev. Lett., 2001, 86 (14):3132-3135.

. Yan Haizhen, Cheng Cheng, Zhang Qinghao. Effect of the temperature on absorption and photoluminescence spectra of CdSe/ZnS quantum dots [J]. Chin. J. Lumin. (发光学报), 2008, 29 (1):166-171 (in Chinese).

Views

208

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Enhancement of Photo-stability of Colloidal CdSe/ZnS Quantum Dots Passivated in SiO₂

Controlled Synthesis and ATP Detection of CuInSe₂ Quantum Dots with Near-infrared Excitation/Emission

Perovskite Based Broadband Photodetector

Rapid Detection of Neonicotinoids by Metal Halide CH₃NH₃PbBr₃ Perovskite Quantum Dots

Current Status and Challenges in Indium Phosphide Quantum Dots and Their Electroluminescence

Related Author

NING Ping-fan

LIU Hong-wei

NIU Ping-juan

LIU Guo-xu

LI Xiao-yun

HAN Li-li

GAO Yi-qun

ZHANG Meiqi

Related Institution

School of Electrical Engineering and Automation, Tianjin Polytechnic University

Engineering Research Center of High Power Solid State Lighting Application System, Ministry of Education, Tianjin Polytechnic University

ShineOn(Beijing) Technology Co. Ltd.

College of Chemistry， Fuzhou University

State Key Laboratory of Structural Chemistry and Fujian Key Laboratory of Nanomaterials， Fujian Institute of Research on the Structure of Matter， Chinese Academy of Sciences

AI问答

Address：No.3888 Dong Nanhu Road, Changchun, Jilin, China Postal code：130033
Tel：0431-86176862 Email：fgxbt@126.com
Technical support is provided by Beijing Founder electronics co., LTD 吉ICP备11002662号-17 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰