Up-converting phosphors material(UPM) is the material that exhibits two-photon anti-Stokes luminescence by up-converting infrared to visible light.Recently there is growing interest in studying UPM because of their most promising use as fluorescent labels for the sensitive detection of cell and tissue antigens.In this field

UPM have many advantages: 1.permanent

strong

anti-Stokes emission of discrete wavelengths;2.unmatched contrast in biological specimens due to the absence of autofluorescence upon excitation with IR light;3.simultaneous detection of multiple target analytes;4.low-cost microscope modifications.When this material is applied to molecular biology

genomic research

virology

and microbiology

it has high potential value in diagnostic pathology

and may offer advantages for the detection of proteins or nucleic acids.Luminescent reporter molecules form an excellent alternative to radioisotopes and are increasingly used in research and clinical diagnostic assays.But in practice

the up-converting phosphors material unmodified have many problems

that is

easy to reunite and deposit

so they are unfit for fluorescent labels reporters directly.Few researches were done to modify this material to improve its stability in water.To enlarge the usage of UPM in fluorescent labels

a kind of up-converting phosphor material(UPM) surface modified with amido-(cyanogen) was prepared through liquid coating-sediment method.With isopropyl as solvent

we used 3-aminopropyltriethoxysilane to modify UPM at a definite temperature and a special substance as catalyst.The spectrum of FT-IR shows the new material has amidocyanogen.TG proves that non-modified material and modified material have different process of losing gravity.SEM finds that the dispersion of the surface modified material is better than that of the non-modified.The experiments show that the stability of the modified UPM is improved.Luminescence test shows that non-modified material and modified material have the same emit peaks but the intensity is much increasing.All these exprements show that a kind of modified UPM with amidocyanogen was prepared successfully.