Up-converting phosphors material(UPM)is the material that exhibits two-photon anti-Stokes luminescence by up-converting infrared to visible light.The material we used is a kind of UCM that emits green light when excited by 980 nm light.In fluorescent labels

UPM have many advantages:1.Permanent

strong

anti-Stokes emission of discrete wavelengths;2.unmatched contrast in biological specimens due to the absence of autofluorescence upon excitation with IR light;3.simultaneous detection of multiple target analytes;4.low-cost microscope modifications.Recently there is growing interest in studying UPM because of their most promising use as fluorescent labels for the sensitive detection of cell and tissue antigens.But the up-converting phosphors materials unmodified are easy to reunite and deposit

so they can't directly apply to fluorescent labels reporters.To enlarge the usages of up-converting phosphors material(UPM)in fluorescent labels and biological check

we prepared a kind of UPM with surface coated by silica and then signified this kind of material.The particle size of the product is very uniform and the particle shape of product keeps spherical.The thickness of silica is about 10 nm and the luminescence intensity has greatly increased.The paper also did a lot of research about the conditions of up-conversion phosphors material cladding by silicon dioxide

and found out the best conditions.We selected n-butyl alcohol as solvent and used ammonia water as dispersing agent.The volume of solvent is 100 mL.The mole ratio is 1:1 between tetraethylorthosilicate and water

and reaction time is 40 min.After the surface coated

the up-conversion phosphors material's dispersibility on aqueous solutions is better than before

and the stability is strengthened.This paper is the first step to modify the UPM

then we will modify the UPM with amino-group.We expect that the new material has better application foreground in biochip field.