Interaction between Transferrin and Adriamycin

YANG Pei-hui; CHEN Xiao-xiang; CAI Ji-ye; ZENG Hui-lan

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Interaction between Transferrin and Adriamycin

更新时间：2020-08-11

- Interaction between Transferrin and Adriamycin
- Chinese Journal of Luminescence Vol. 26, Issue 5, Pages: 669-673(2005)
- 作者机构：
  
  1. 暨南大学, 化学系,广东广州,510630
  2. 暨南大学, 医学院第一附属医院,广东广州,510630
- 作者简介：
- 基金信息：
- DOI：
  CLC： O482.31;O657.32
- Received：27 January 2005，
  
  Revised：2005-6-22，
  
  Published：20 September 2005
- 稿件说明：
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杨培慧, 陈晓翔, 蔡继业, 曾慧兰. 转铁蛋白载体携带阿霉素的分子间结合作用[J]. 发光学报, 2005,26(5): 669-673

YANG Pei-hui, CHEN Xiao-xiang, CAI Ji-ye, ZENG Hui-lan. Interaction between Transferrin and Adriamycin[J]. Chinese Journal of Luminescence, 2005,26(5): 669-673
杨培慧, 陈晓翔, 蔡继业, 曾慧兰. 转铁蛋白载体携带阿霉素的分子间结合作用[J]. 发光学报, 2005,26(5): 669-673 DOI：

YANG Pei-hui, CHEN Xiao-xiang, CAI Ji-ye, ZENG Hui-lan. Interaction between Transferrin and Adriamycin[J]. Chinese Journal of Luminescence, 2005,26(5): 669-673 DOI：

摘要

利用荧光光谱法和吸收光谱法研究阿霉素与转铁蛋白间的结合作用

确定了阿霉素对转铁蛋白的荧光猝灭过程的猝灭机理

测定了不同浓度下该结合反应的结合常数

=(0.98~89.1)×10

结合位点数

=1.09~1.37

并依据能量转移理论确定了药物和蛋白间的结合距离

=1.94nm。

Abstract

To understand the interaction between adriamycin(ADR) and transferrin(Tf) solution

the binding reaction between ADR and Tf was investigated by fluorescence and ultraviolet visible Absorption(UV-Vis) spectroscopy methods.The mechanism of the fluorescence quenching of Tf induced by ADRwas determined.The relation between concentration of ADR and

was linear.It showed that the quenching of Tf induced by ADR was either dynamic or static quenching.The ultraviolet Absorption of Tf decreasing in the presence of ADR showed that a nonluminous compound was formed by the interaction of Tf and ADR.So it was confirmed that the mechanism of the fluorescence quenching of Tf induced by ADR was static quenching. Furthermore

we use Stern-Volmer equation to approve it.The equilibrium constants

were measured equaling to(0.98～89.1)×10

while the numbers of binding sites n were 1.09～1.37 at different concentration by means of fluorescence quenching method.The binding distance

=1.94 nm in the interaction of 6.9×10

-5

mol/L ADRand 5.6×10

-6

mol/L Tf was also obtained according to Frster theory of non-radiation energy transfer.Akind of binary compound of ADRand Tf was formed

providing the instructional meaning for the preparation of oriented drug carried by Tf.

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