The metabolic level of medications which treat cancer was studied by fluorescence spectroscopic analysis in the patients body

the medications were quantitatively analyzed in the urine that was simulated with water as solvent. The measuring conditions were discussed

including the effects of fluorescence quenching

sensitization medium and the medication concentration change. The method of quantitative analysis was researched for the medication metabolizing in body by fluorescence spectroscopy

and the sensitizing pattern between donor and acceptor of energy was discussed with Förster theory in this paper. The results show that fluorescence is quenched as the compound containing Fe

#

concentration is 1×10

-4

g/mL in 1×10

-7

g/mL HPD (hematoporphyrin); the luminescence intensity of poxorubicin hydrochloride is higher as its concentration is lower from contrasting concentration 1×10

-3

g/mL to 1×10

-4

g/mL for fluorescence and excitation spectra

respectively

and the spectra are shifting red or blue when the concentration quenching arise

then can get rid of the interference of concentration effect by changing the point of measuring luminescence intensity on the line area

and the fluorescence and excitation spectra of Poxorubicin Hydrochloride; Etoposide; Vincristinal Sulfas and Mitomycin-c for injection could also be obtained from this experiment. The detection limit of this method can achieve 10

-3

～10

-7

g/mL

and this results show that detection sensitivity of fluorescence spectroscopy is higher than those of other analytic methods.