STUDY ON THE CHEMILUMINESCENTPROPERTIES OF ETHIDIUM BROMIDE

ZHUANG Huisheng; CHEN Guonan; HUANG Jinling

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STUDY ON THE CHEMILUMINESCENTPROPERTIES OF ETHIDIUM BROMIDE

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- STUDY ON THE CHEMILUMINESCENTPROPERTIES OF ETHIDIUM BROMIDE
- Chinese Journal of Luminescence Vol. 20, Issue 4, Pages: 358-362(1999)
- 作者机构：
  
  福州大学化学系, 福建福州350002
- 作者简介：
- 基金信息：
- DOI：
  CLC： O644.19
- Received：02 November 1998，
  
  Published：30 November 1999
- 稿件说明：
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庄惠生, 陈国南, 黄金陵. 溴乙锭的化学发光性质研究[J]. 发光学报, 1999,20(4): 358-362

ZHUANG Huisheng, CHEN Guonan, HUANG Jinling. STUDY ON THE CHEMILUMINESCENTPROPERTIES OF ETHIDIUM BROMIDE[J]. Chinese Journal of Luminescence, 1999,20(4): 358-362
庄惠生, 陈国南, 黄金陵. 溴乙锭的化学发光性质研究[J]. 发光学报, 1999,20(4): 358-362 DOI：

ZHUANG Huisheng, CHEN Guonan, HUANG Jinling. STUDY ON THE CHEMILUMINESCENTPROPERTIES OF ETHIDIUM BROMIDE[J]. Chinese Journal of Luminescence, 1999,20(4): 358-362 DOI：

摘要

首次研究了澳乙锭的化学发光性质

发现在强酸性介质中

RNA能增强澳乙锭与高锰酸钾反应的化学发光强度。在EC-KMnO

-RNA体系中获得的化学发光强度与RNA浓度在一定范围内成线性关系

因此

建立了一种测定RNA的化学发光分析新方法。并用于合成样品中的RNA含量的测定。方法的线性范围为0.10～10.0μm/mL

检测限为0.060μg/mL

测定浓度为3.5μg/mL的RNA溶液12次

求得相对标准偏差为3.8%;具有较高的精密度

准确度和选择性。

Abstract

In this work

we first investigated the chemiluminescent properties of ethidiumbromide

and find that RNA can enhance the chemiluminescent intensity of reaction between ethidium bromide and KMnO

in a strong acid medium. There is a linear relationship between the chemiluminescent intensity obtained in the system of EB-KMnO

-RNA and RNA concentration

therefore

a new chemiluminescent analytical method for thedetermination of RNA is established and used to determine RNA in synthetic samples.The linear calibration ranges is 0.10~10.0μg/mL and the detection limit is 0.06μg/mL for RNA

the relative standard deviation is 3.8% for 3.5μg/mL RNA. Interference ionswere examined. The precision

accuracy and selectivity of the method are satisfactory.

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