OPTIMAL PARAMETERS OF MEASURING TESTOSTERONE-3-ACRIDINIUM ESTER AND ANTIBODY TITER FOR CHEMILUMINESCENCE IMMUNOASSAY
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OPTIMAL PARAMETERS OF MEASURING TESTOSTERONE-3-ACRIDINIUM ESTER AND ANTIBODY TITER FOR CHEMILUMINESCENCE IMMUNOASSAY
Chinese Journal of LuminescenceVol. 16, Issue 1, Pages: 88-94(1995)
作者机构:
国家核医学重点实验室, 江苏省原子医学研究所 无锡,214063
作者简介:
基金信息:
DOI:
CLC:
Published:28 February 1995,
Received:07 June 1994,
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JIN JIAN, LUO SHINENG, WANG BOCHENG, et al. OPTIMAL PARAMETERS OF MEASURING TESTOSTERONE-3-ACRIDINIUM ESTER AND ANTIBODY TITER FOR CHEMILUMINESCENCE IMMUNOASSAY. [J]. Chinese journal of luminescence, 1995, 16(1): 88-94.
DOI:
JIN JIAN, LUO SHINENG, WANG BOCHENG, et al. OPTIMAL PARAMETERS OF MEASURING TESTOSTERONE-3-ACRIDINIUM ESTER AND ANTIBODY TITER FOR CHEMILUMINESCENCE IMMUNOASSAY. [J]. Chinese journal of luminescence, 1995, 16(1): 88-94.DOI:
OPTIMAL PARAMETERS OF MEASURING TESTOSTERONE-3-ACRIDINIUM ESTER AND ANTIBODY TITER FOR CHEMILUMINESCENCE IMMUNOASSAY
In order to developing sensitive and precise testoterone chemiluminescence immunoassdy
optimal paremeters of measuring acridinium ester and antibody titer must be selected.In this study
we find that time paremeter for luminescence analysis testosterone-3-carboxymethyloxime-4-(To-mehty)-acridinium-9-carboxylate(To-3AEPMAC)is very important in the low concentration.During the measurement
there is a peak of solvent in 0.00~0.21s
peak of To-3-AEPMAC in 0.43~2.22s.The luminescence reaction can be completed in 0.32~3.91s.But it is not very correction because the more To-3-AEPMAC is
the longer luminescence peak time is.These results are decided by the reaction mixing condition and reactive dynamics.The solvent peak occupies 4.10±2.21%(
x
±s)in all luminescence.The same result is obtained with measuing the reaction rate.It is best to choose 0.30 s delay time and 10 s integrated time for analysis the chemiluminescence characteristic.This measuring manner riot only refused the luminescence disturbance of solvent
but also obtained all signal of To-3-AEPMAC.Then it is bad to analysis the hight peak of luminescence because of varying time in peak appearing.Optimal antibody titer is measured with three methods
there are:maximum value of antibody diluted curve
maximum verying reaction and minimum reaction standard error.The first and second methods are respected for sensition
the later method is respected for precision of standard curve.The optimal antibody titer of testosterone chemiluminescence immunoassay is 1:18000.