LIPID MEMBRANE PEROXIDATION MEASURED BY CHEMILUMINESCENCE (CL)

Hu Tianxi; Xia Jiazhi; Wu Xiaohua; Ma Yuanming

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LIPID MEMBRANE PEROXIDATION MEASURED BY CHEMILUMINESCENCE (CL)

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- LIPID MEMBRANE PEROXIDATION MEASURED BY CHEMILUMINESCENCE (CL)
- Chinese Journal of Luminescence Vol. 7, Issue 1, Pages: 99-102(1986)
- 作者机构：
  
  1. 华北师范大学生物系
  2. 上海医科大学约物系
- 作者简介：
- 基金信息：
- DOI：
  CLC：
- Published：28 February 1986
- 稿件说明：
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胡天喜, 夏嘉治, 吴筱华, 马远呜. 用化学发光法测量脂膜过氧化[J]. 发光学报, 1986,7(1): 99-102

Hu Tianxi, Xia Jiazhi, Wu Xiaohua, Ma Yuanming. LIPID MEMBRANE PEROXIDATION MEASURED BY CHEMILUMINESCENCE (CL)[J]. Chinese Journal of Luminescence, 1986,7(1): 99-102
胡天喜, 夏嘉治, 吴筱华, 马远呜. 用化学发光法测量脂膜过氧化[J]. 发光学报, 1986,7(1): 99-102 DOI：

Hu Tianxi, Xia Jiazhi, Wu Xiaohua, Ma Yuanming. LIPID MEMBRANE PEROXIDATION MEASURED BY CHEMILUMINESCENCE (CL)[J]. Chinese Journal of Luminescence, 1986,7(1): 99-102 DOI：

摘要

血清或血浆

各类生物细胞和亚细胞器

普遍存在着自发的、低水平的化学发光现象

一般认为

这类发光是脂质过氧化所引起来的。为了搞清这种现象的本质

本试验选用了平面脂膜、人工囊泡沫—脂质体及生物膜—红细胞影泡为材料

使用了液体闪烁计数器测量脂质的氧化发光

从而以发光强度为准

判定脂膜过氧化的程度。

Abstract

Lipid Membrane peroxidation of plane lipid membrane

liposome

red blood cell ghost peroxidation was investigated by chemiluminescence (CL)

The results were showed as follows:1. There are lipid peroxidation and spontaneous CL on the lipid membrane. The peroxidation and CL intensity related to the content of unsatu rated fatty acid. CL was enhanced by physical and chemical factors.2. The lipid membrane peroxidation depended on temperature

PO2 and lipid concentration in its medium. These factors should be carefully controlled during experiments.3. Utraweak lipid peroxidation could be measured by CL

CL measurement with liquid scintillation counter (L.S.C.) is a more sensitive

faster and simpler method.

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