The bioluminescent spectra of six species of marine luminous bacteria in vivo were reserched. They are Vibrio orientalis

V.fischeri

V

splendi-dus biotype Ⅰ

Ⅴ.harvyi

Photobacterium phosphoreum and P.leiognathi. Two strains were selected from each of these species respectively for this studying. It was found that their maximun emission peaking of biolumines cence spectra were between 474-485nm. All λ

max

of Vibrio spp. were bet ween 482-485nm

and all λ

max

of Photobacterium spp. were between 474-480nm. The bioluminescence spectra of Vibrio were different from Pbolcba-cterium. The maximun emission peaking of spectra of Viobrio orientalis were at 484±1nm.