The chlorophyll in non-polar solvent could aggregated into dimer or polymers. The aggregated chlorophyll in petroleum ether (mainly polymers) could emit faint light for a few minutes after illumination. The decay curves of the delay light emission (DLE) showed two phases:fast phase which lasted for about ten seconds and slow phase which lasted for about several minutes. The intensity of the fast phase would increase slightly when the illumination time was sustained more than one minute
while the intensity of slow phase would increase continuously
The decay curves of the DLE of aggregated chlorophyll in CC1
4
(mainly dimers) was different from that in petroleum ether:the intensity of the slow phase was reduced obviously
while the intensity of the fast phase was as same as that in petroleum ether. The intensity of the DLE was lowered by adding electronic donor or accepter When the aggregated chlorophyll was disaggregated
there was no DLE. It was evident that light energy could store up in the molecules of chlorophyll aggregates.